Testing Data
Rabbit anti-Human CD31 Monoclonal Antibody | anti-CD31 antibody
Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purified using protein A
Synonyms
CD31; Monoclonal Antibody; Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody; EndoCAM; GPIIA; PECA1; CD_antigen: CD31; PECAM-1; anti-CD31 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
RM247
Purity/Purification
Affinity purified using protein A
Form/Format
Colorless liquid
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Sequence Length
738
Applicable Applications for anti-CD31 antibody
Immunohistochemistry (IHC); Western Blot (WB)
Application Notes
IHC: 1:1000-1:2500 dilution
WB: 1:1000- 1:2000 dilution
WB: 1:1000- 1:2000 dilution
Immunogen
A peptide corresponding to the cytoplasmic domain of human CD31
Preparation and Storage
Store at -20 degree C for 12 months.
The antibody solution should be gently mixed before use.
The antibody solution should be gently mixed before use.
Testing Data
Testing Data
Related Product Information for anti-CD31 antibody
Rabbit monoclonal antibody to detect CD31 in human samples.
Induces susceptibility to atherosclerosis. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals; and changes function on apoptosis; promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis; the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte; thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
Induces susceptibility to atherosclerosis. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals; and changes function on apoptosis; promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis; the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte; thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
81,378 Da
NCBI Official Full Name
platelet endothelial cell adhesion molecule
UniProt Protein Name
Platelet endothelial cell adhesion molecule
UniProt Gene Name
PECAM1
UniProt Synonym Gene Names
PECAM-1
Uniprot Description
PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.
Protein type: Cell adhesion; Cell development/differentiation; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q23.3
Cellular Component: extracellular space; plasma membrane; platelet alpha granule membrane; secretory granule membrane
Molecular Function: protein binding
Biological Process: cell recognition; diapedesis; extracellular matrix organization and biogenesis; leukocyte migration; neutrophil degranulation; phagocytosis; platelet degranulation; signal transduction
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Product Notes
The CD31 pecam1 (Catalog #AAA846001) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD31 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC); Western Blot (WB). IHC: 1:1000-1:2500 dilution WB: 1:1000- 1:2000 dilution. Researchers should empirically determine the suitability of the CD31 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD31, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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