Goat Low-density lipoprotein-receptor-related protein 6 ELISA Kit | LRP6 elisa kit

Goat Low-density lipoprotein-receptor-related protein 6 ELISA Kit

Cat. Num. AAA738371

• Gene Names: LRP6; ADCAD2
• Reactivity: Goat
• Synonyms: Low-density lipoprotein-receptor-related protein 6; Goat Low-density lipoprotein-receptor-related protein 6 ELISA Kit; LRP6 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LRP6 elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This LRP6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat LRP6. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY LRP6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LRP6 antibody and an LRP6-HRP conjugate. The assay sample and buffer are incubated together with LRP6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LRP6 concentration since LRP6 from samples and LRP6-HRP conjugate compete for the anti-LRP6 antibody binding site. Since the number of sites is limited, as more sites are occupied by LRP6 from the sample, fewer sites are left to bind LRP6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LRP6 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for LRP6 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 148727288
• NCBI GeneID: 4040
• NCBI Accession #: NP_002327.2
• NCBI GenBank Nucleotide #: NM_002336.2
• UniProt Accession #: O75581; Q17RZ2; Q59EX8; Q9UQH4
• Molecular Weight: 180,429 Da
• NCBI Official Full Name: low-density lipoprotein receptor-related protein 6
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein 6
• NCBI Official Symbol: LRP6
• NCBI Official Synonym Symbols: ADCAD2
• NCBI Protein Information: low-density lipoprotein receptor-related protein 6; LRP-6
• UniProt Protein Name: Low-density lipoprotein receptor-related protein 6
• Protein Family: Low-density lipoprotein receptor-related protein
• UniProt Gene Name: LRP6
• UniProt Synonym Gene Names: LRP-6
• UniProt Entry Name: LRP6_HUMAN

NCBI Description

This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.[provided by RefSeq, Dec 2009]

Uniprot Description

LRP6: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation. Homodimer; disulfide-linked. Forms phosphorylated oligomer aggregates on Wnt-signaling. Forms a WNT-signaling complex formed of a WNT protein, a FZD protein and LRP5 or LRP6. Interacts (via the extracellular domain) with WNT1; the interaction is enhanced by prior formation of the Wnt/Fzd complex. Interacts (via the beta-propeller regions 3 and 4) with WNT3A. Interacts (via the beta-propeller regions 1 and 2) with WNT9B. Interacts with FZD5; the interaction forms a coreceptor complex for Wnt signaling and is inhibited by DKK1 and C1orf187. Interacts (via beta propeller region) with DKK1; the interaction inhibits FZD5/LRP6 complex formation. Interacts with DKK2. Interacts with C1orf187/DRAXIN; the interaction inhibits Wnt signaling. Interacts (via the phosphorylated PPPSP motifs) with AXIN1; the interaction recruits the AXIN1/GSK3B complex to cell surface LRP6 signalsomes. Interacts with GRB10; the interaction prevents AXIN1 binding, thus negatively regulating the Wnt signaling pathway. Interacts (via the extracellular domain) with RSPO1; the interaction activates Wnt/beta-catenin signaling. Interacts (via the extracellular domain) with RSPO3 (via the cysteine rich domain); the interaction activates Wnt/beta-catenin signaling. Interacts (via the beta- propeller regions 1 and 2) with SOST; the interaction competes with DKK1 for binding for inhibiting beta-catenin signaling. Interacts with MESD; the interaction prevents the formation of LRP6 aggregates and targets LRP6 to the plasma membrane. Interacts (via the cytoplasmic domain) with CSNKIE; the interaction phosphorylates LRP6, binds AXIN1 and inhibits AXIN1/GSK3B-mediated phosphorylation of beta-catenin. Interacts with MACF1. Decreased levels on WNT3A stimulation. Widely co-expressed with LRP5 during embryogenesis and in adult tissues. Belongs to the LDLR family.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; early endosome; integral to membrane; extracellular region; caveola; cell soma; early endosome membrane; plasma membrane; synapse; cytoplasmic vesicle; receptor complex

Molecular Function: low-density lipoprotein receptor activity; identical protein binding; Wnt-protein binding; Wnt receptor activity; protein binding; protein homodimerization activity; frizzled binding; kinase inhibitor activity; toxin transporter activity; apolipoprotein binding; receptor binding

Biological Process: embryonic forelimb morphogenesis; response to peptide hormone stimulus; radial glial cell differentiation in the forebrain; positive regulation of apoptosis; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; cerebellum morphogenesis; thalamus development; palate development; Wnt receptor signaling pathway through beta-catenin; bone remodeling; embryonic pattern specification; embryonic hindlimb morphogenesis; Wnt receptor signaling pathway in forebrain neuroblast division; anterior/posterior pattern formation; synaptic transmission; cell-cell adhesion; elevation of cytosolic calcium ion concentration; negative regulation of protein amino acid phosphorylation; convergent extension; response to folic acid; positive regulation of mesenchymal cell proliferation; midbrain development; positive regulation of bone resorption; neural crest cell differentiation; heart looping; cell migration involved in gastrulation; negative regulation of epithelial cell proliferation; embryonic limb morphogenesis; Wnt receptor signaling pathway; external genitalia morphogenesis; neural crest formation; positive regulation of cell cycle; gastrulation with mouth forming second; negative regulation of fat cell differentiation; positive regulation of ossification; odontogenesis of dentine-containing teeth; formation of radial glial scaffolds; cerebral cortex cell migration; embryonic retina morphogenesis in camera-type eye; neural tube closure; midbrain-hindbrain boundary development; negative regulation of protein kinase activity; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; embryonic digit morphogenesis; cerebral cortex development; regulation of ossification

Disease: Coronary Artery Disease, Autosomal Dominant 2