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Western Blot (WB) (Western Blot analysis of mouse liver tissue lysates (35ug/lane) usingMBS625955. CD302 (arrow) was detected usingMBS625955.)

Rabbit anti-Human, Mouse CD302 Polyclonal Antibody | anti-CD302 antibody

CD302, ID (CD302 Antigen, C-type Lectin Domain Family 13 Member A, C-type Lectin BIMLEC, Type I Transmembrane C-type Lectin Receptor DCL-1, KIAA0022, DCL1, CLEC13A) (Biotin)

Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot, Immunohistochemistry
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CD302; Polyclonal Antibody; ID (CD302 Antigen; C-type Lectin Domain Family 13 Member A; C-type Lectin BIMLEC; Type I Transmembrane C-type Lectin Receptor DCL-1; KIAA0022; DCL1; CLEC13A) (Biotin); anti-CD302 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CD302. Species Crossreactivity: mouse
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Sequence Length
232
Applicable Applications for anti-CD302 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 143-170 in the central region of human CD302 (NP_055695; Q8IX05). Cellular Localization: Membrane. Cell projection, filopodium. Cytoplasm, cell cortex.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse liver tissue lysates (35ug/lane) usingMBS625955. CD302 (arrow) was detected usingMBS625955.)

Western Blot (WB) (Western Blot analysis of mouse liver tissue lysates (35ug/lane) usingMBS625955. CD302 (arrow) was detected usingMBS625955.)

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin-fixed and paraffin-embedded human hepatocarcinoma usingMBS625955, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS625955 for immunohistochemistry.)

Immunohistochemistry (IHC) (Immunohistochemistry of formalin-fixed and paraffin-embedded human hepatocarcinoma usingMBS625955, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS625955 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of HepG2 cells (bottom histogram) using MBS625955 compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow Cytometry analysis of HepG2 cells (bottom histogram) using MBS625955 compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CD302 antibody
CD302 is a C-type lectin receptor involved in cell adhesion and migration, as well as endocytosis and phagocytosis.
Product Categories/Family for anti-CD302 antibody
References
1. Kato, M., J. Immunol. 179(9), 6052-6063 (2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26.183
NCBI Official Full Name
CD302 antigen isoform 1
UniProt Protein Name
CD302 antigen
Protein Family
UniProt Gene Name
CD302
UniProt Synonym Gene Names
CLEC13A; DCL1; KIAA0022
UniProt Entry Name
CD302_HUMAN

Uniprot Description

CD302: Potential multifunctional C-type lectin receptor that may play roles in endocytosis and phagocytosis as well as in cell adhesion and migration. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: microvillus; membrane; integral to membrane; cell cortex; filopodium

Molecular Function: carbohydrate binding

Biological Process: phagocytosis

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Product Notes

The CD302 cd302 (Catalog #AAA6468304) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD302, ID (CD302 Antigen, C-type Lectin Domain Family 13 Member A, C-type Lectin BIMLEC, Type I Transmembrane C-type Lectin Receptor DCL-1, KIAA0022, DCL1, CLEC13A) (Biotin) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD302 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD302 cd302 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD302, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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