Typical Testing Data/Standard Curve (for reference only)
Human CD302 antigen (CD302) ELISA Kit | CD302 elisa kit
Human CD302 antigen (CD302) ELISA Kit
Gene Names
CD302; DCL1; DCL-1; BIMLEC; CLEC13A
Reactivity
Human
Synonyms
CD302 antigen (CD302); Human CD302 antigen (CD302) ELISA Kit; CD302 elisa kit
Reactivity
Human
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CD302 elisa kit
Intended Uses: This CD302 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD302. This ELISA kit for research use only!
Principle of the Assay: CD302 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CD302 antibody and an CD302-HRP conjugate. The assay sample and buffer are incubated together with CD302-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD302 concentration since CD302 from samples and CD302-HRP conjugate compete for the anti-CD302 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD302 from the sample, fewer sites are left to bind CD302-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD302 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CD302 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CD302 antibody and an CD302-HRP conjugate. The assay sample and buffer are incubated together with CD302-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD302 concentration since CD302 from samples and CD302-HRP conjugate compete for the anti-CD302 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD302 from the sample, fewer sites are left to bind CD302-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD302 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CD302 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
215,781 Da
NCBI Official Full Name
CD302 antigen isoform 1
NCBI Official Synonym Full Names
CD302 molecule
NCBI Official Symbol
CD302
NCBI Official Synonym Symbols
DCL1; DCL-1; BIMLEC; CLEC13A
NCBI Protein Information
CD302 antigen; DEC205-associated C-type lectin 1; C-type lectin domain family 13, member A; type I transmembrane C-type lectin receptor DCL-1
UniProt Protein Name
CD302 antigen
Protein Family
UniProt Gene Name
CD302
UniProt Synonym Gene Names
CLEC13A; DCL1; KIAA0022
UniProt Entry Name
CD302_HUMAN
NCBI Description
CD302 is a C-type lectin receptor involved in cell adhesion and migration, as well as endocytosis and phagocytosis (Kato et al., 2007 [PubMed 17947679]).[supplied by OMIM, Aug 2008]
Uniprot Description
CD302: Potential multifunctional C-type lectin receptor that may play roles in endocytosis and phagocytosis as well as in cell adhesion and migration. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q24.2
Cellular Component: microvillus; membrane; integral to membrane; cell cortex; filopodium
Molecular Function: carbohydrate binding
Biological Process: phagocytosis
Research Articles on CD302
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Product Notes
The Human CD302 cd302 (Catalog #AAA7249512) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7249512 ELISA Kit recognizes Human CD302. It is sometimes possible for the material contained within the vial of "CD302 antigen (CD302), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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