Typical Testing Data/Standard Curve
Human Sodium/potassium transporting ATPase subunit Alpha 2 (ATP1A2) ELISA Kit | ATP1A2 elisa kit
Human Sodium/potassium transporting ATPase subunit Alpha 2 (ATP1A2) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||ATP1A2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ATP1A2 antibody and an ATP1A2-HRP conjugate. The assay sample and buffer are incubated together with ATP1A2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP1A2 concentration since ATP1A2 from samples and ATP1A2-HRP conjugate compete for the anti-ATP1A2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP1A2 from the sample, fewer sites are left to bind ATP1A2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP1A2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 2 subunit. Mutations in this gene result in familial basilar or hemiplegic migraines, and in a rare syndrome known as alternating hemiplegia of childhood. [provided by RefSeq, Oct 2008]
Uniprot Description
ATP1A2: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients. Defects in ATP1A2 are the cause of familial hemiplegic migraine type 2 (FHM2). FHM2 is a rare, severe, autosomal dominant subtype of migraine characterized by aura and some hemiparesis. Defects in ATP1A2 are a cause of alternating hemiplegia of childhood 1 (AHC1). AHC is typically distinguished from familial hemiplegic migraine by infantile onset of the symptoms and high prevalence of associated neurological deficits that become increasingly obvious with age. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
Protein type: EC 3.6.3.9; Membrane protein, integral; Transporter; Transporter, ion channel; Hydrolase; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1q23.2
Cellular Component: T-tubule; cytoplasm; plasma membrane; dendritic spine; synapse; caveola; sodium:potassium-exchanging ATPase complex; vesicle; endosome
Molecular Function: metal ion binding; chaperone binding; sodium:potassium-exchanging ATPase activity; ATP binding
Biological Process: regulation of smooth muscle contraction; response to nicotine; cellular sodium ion homeostasis; negative regulation of heart contraction; adult locomotory behavior; metabolic process; regulation of striated muscle contraction; sodium ion transport; neurological control of breathing; cellular potassium ion homeostasis; negative regulation of striated muscle contraction; potassium ion import; regulation of vasoconstriction; regulation of blood pressure; reduction of cytosolic calcium ion concentration; ATP hydrolysis coupled proton transport; neurotransmitter uptake; visual learning; locomotion; transmembrane transport; regulation of the force of heart contraction; potassium ion transport; cardiac muscle contraction
Disease: Migraine, Familial Hemiplegic, 2; Alternating Hemiplegia Of Childhood 1
Research Articles on ATP1A2
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Product Notes
The Human ATP1A2 atp1a2 (Catalog #AAA7240961) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7240961 ELISA Kit recognizes Human ATP1A2. It is sometimes possible for the material contained within the vial of "Sodium/potassium transporting ATPase subunit Alpha 2 (ATP1A2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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