Rabbit anti-Human CD28 Polyclonal Antibody | anti-CD28 antibody

CD28, CT (CD28, T-cell-specific surface glycoprotein CD28, TP44, CD28) (FITC)

Cat. Num. AAA6283008

• Gene Names: CD28; Tp44
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD28; Polyclonal Antibody; CT (CD28; T-cell-specific surface glycoprotein CD28; TP44; CD28) (FITC); anti-CD28 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CD28 antibody: Immunofluorescence (IF), FLISA, Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188~217 amino acids from the C-terminal region of human CD28.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CD28 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). CD28 (arrow) was detected using the purified Pab.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CD28 Antibody (C-term) with Hela cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-CD28 antibody

CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.

Product Categories/Family for anti-CD28 antibody

Antibodies; CD Markers

References

Zocchi, M.R., et.al., Eur. J. Cancer 28A (4-5), 749-754 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 340545508
• NCBI GeneID: 940
• NCBI Accession #: NP_001230006.1
• NCBI GenBank Nucleotide #: NM_001243077.1
• UniProt Accession #: P10747; Q13964; Q52M23; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
• Molecular Weight: 25,066 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 2
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28; CD28 antigen
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_HUMAN

NCBI Description

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to plasma membrane; plasma membrane; immunological synapse; cytosol; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; protease binding; coreceptor activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of translation; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine biosynthetic process; T cell receptor signaling pathway; positive regulation of inflammatory response to antigenic stimulus; cell surface receptor linked signal transduction; positive regulation of T cell proliferation; regulatory T cell differentiation; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of viral genome replication; positive regulation of mitosis; negative thymic T cell selection; regulation of defense response to virus by virus; humoral immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; T cell costimulation; innate immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of transcription from RNA polymerase II promoter