Rabbit anti-Mouse, Rat CD273 Polyclonal Antibody | anti-CD273 antibody

RABBIT ANTI HUMAN CD273

Cat. Num. AAA223722

• Gene Names: PDCD1LG2; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: CD273; Polyclonal Antibody; RABBIT ANTI HUMAN CD273; anti-CD273 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects human CD273, also known as B7-DC and Programmed Death Ligand 2 (PD-L2). CD273 is a single-pass type 1 membrane protein belonging to the immunoglobulin receptor superfamily, and is a ligand for CD279 (PD-1). ; ; CD273 is highly expressed in the tissues of the heart, liver, lung, placenta and pancreas. It is expressed in dendritic cells, and its expression can be induced on inflammatory macrophages when activated by interleukin 4 or Th2 cells. ; ; CD273 functions to inhibit T-cell proliferation by blocking cell cycle progression and cytokine production, and is particularly important in the maintenance of self tolerance.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 273

• Applicable Applications for anti-CD273 antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 30kDa in Raji cell lysate; Immunohistology - Paraffin: Maximum Dilution: 2.5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: A peptide corresponding to a 16 amino acid sequence from near the centre of Human CD273.; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to Human CD273 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from Raji, human Burkitt's lymphoma cells probed with Rabbit anti Human CD273 (MBS223722) at 0.5(A), 1(B) and 2(C) ug/ml)

Testing Data #2

(Immunohistochemical staining of mouse brain with Rabbit anti Human CD273 (MBS223722))

Related Product Information for anti-CD273 antibody

MBS223722 detects human CD273, also known as B7-DC and Programmed Death Ligand 2 (PD-L2). CD273 is a single-pass type 1 membrane protein belonging to the immunoglobulin receptor superfamily, and is a ligand for CD279 (PD-1). CD273 is highly expressed in the tissues of the heart, liver, lung, placenta and pancreas. It is expressed in dendritic cells, and its expression can be induced on inflammatory macrophages when activated by interleukin 4 or Th2 cells. CD273 functions to inhibit T-cell proliferation by blocking cell cycle progression and cytokine production, and is particularly important in the maintenance of self tolerance.

NCBI and Uniprot Product Information

• NCBI GI #: 190014605
• NCBI GeneID: 80380
• NCBI Accession #: NP_079515.2
• NCBI GenBank Nucleotide #: NM_025239.3
• UniProt Accession #: Q9BQ51; Q14CN8; Q5T7Z6; Q6JXL8; Q6JXL9
• Molecular Weight: 20,745 Da
• NCBI Official Full Name: programmed cell death 1 ligand 2
• NCBI Official Synonym Full Names: programmed cell death 1 ligand 2
• NCBI Official Symbol: PDCD1LG2
• NCBI Official Synonym Symbols: B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
• NCBI Protein Information: programmed cell death 1 ligand 2; B7 dendritic cell molecule; B7-DC; PD-1 ligand 2; PD-1-ligand 2; PDCD1 ligand 2; butyrophilin B7-DC; programmed death ligand 2
• UniProt Protein Name: Programmed cell death 1 ligand 2
• UniProt Gene Name: PDCD1LG2
• UniProt Synonym Gene Names: B7DC; CD273; PDCD1L2; PDL2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; B7-DC
• UniProt Entry Name: PD1L2_HUMAN

Uniprot Description

PDCD1LG2: Involved in the costimulatory signal, essential for T- cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24.2

Cellular Component: integral to membrane; plasma membrane; endomembrane system

Biological Process: negative regulation of interleukin-10 production; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; T cell costimulation; positive regulation of T cell proliferation; immune response