Mouse Alpha-crystallin B chain ELISA Kit | Cryab elisa kit

Mouse Alpha-crystallin B chain ELISA Kit

Cat. Num. AAA762806

• Gene Names: CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
• Reactivity: Mouse
• Synonyms: Alpha-crystallin B chain; Mouse Alpha-crystallin B chain ELISA Kit; Cryab/CRYA2/CTPP2/HSPB5/alpha B crystallin/alpha (B)-crystallin/Alpha (B)-crystallin/CRYA2alpha crystallin B chain/crystallin; alpha B/CTPP2/Heat shock protein beta-5/heat-shock 20 kD like-protein/HspB5/HSPB5/Renal carcinoma antigen NY-REN-27/Rosenthal fiber component; Cryab elisa kit

• Reactivity: Mouse
• Sequence Length: 175

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Cryab elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Cryab antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Cryab antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Cryab amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Cryab can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 577019573
• NCBI GeneID: 1410
• NCBI Accession #: NP_001276737.1
• NCBI GenBank Nucleotide #: NM_001289808.1
• Molecular Weight: 20,159 Da
• NCBI Official Full Name: alpha-crystallin B chain
• NCBI Official Synonym Full Names: crystallin alpha B
• NCBI Official Symbol: CRYAB
• NCBI Official Synonym Symbols: MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
• NCBI Protein Information: alpha-crystallin B chain
• UniProt Protein Name: Alpha-crystallin B chain
• Protein Family: Alpha-crystallin
• UniProt Gene Name: CRYAB
• UniProt Synonym Gene Names: CRYA2; HSPB5; HspB5
• UniProt Entry Name: CRYAB_HUMAN

NCBI Description

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

CRYAB: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP also known as the HSP20) family. Alpha-B is expressed in the lens as well as other tissues. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 11q22.3-q23.1

Cellular Component: actin filament bundle; cell surface; cytoplasm; cytosol; Golgi apparatus; microtubule cytoskeleton; mitochondrion; myelin sheath; nucleoplasm; nucleus; plasma membrane; Z disc

Molecular Function: identical protein binding; metal ion binding; microtubule binding; protein binding; protein homodimerization activity; structural constituent of eye lens; unfolded protein binding

Biological Process: aging; lens development in camera-type eye; microtubule polymerization or depolymerization; muscle contraction; muscle development; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of cell growth; negative regulation of intracellular transport; protein folding; protein homooligomerization; response to estradiol stimulus; response to hydrogen peroxide; response to hypoxia; stress-activated MAPK cascade; tubulin folding

Disease: Cardiomyopathy, Dilated, 1ii; Cataract 16, Multiple Types; Myopathy, Myofibrillar, 2; Myopathy, Myofibrillar, Fatal Infantile Hypertonic, Alpha-b Crystallin-related