PD-L2 Active Protein | PDCD1LG2 active protein

PD-L2 Protein, Human, Recombinant (ECD, His Tag)

Cat. Num. AAA8120169

• Gene Names: PDCD1LG2; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
• Purity: >98% as determined by SDS-PAGE
• Synonyms: PD-L2; PD-L2 Protein; Human; Recombinant (ECD; His Tag); Human B7-DC/PD-L2/CD273 Protein (His Tag); B7-DC Protein; B7DC Protein; bA574F11.2 Protein; Btdc Protein; CD273 Protein; PDCD1L2 Protein; PDL2 Protein; programmed cell death 1 ligand 2; PDCD1LG2 active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro219

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 20
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Pro 219) of human PD-L2 (NP_079515.2) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human PD-L2 at 2ug/ml (100uL/well) can bind human PD-1. The EC50 of human PD-1 is 150-800ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human PD-L2 at 2 ug/ml (100 ul/well) can bind human PD-1, the EC50 of human PD-1 is 150-800 ng/mL.)

Related Product Information for PDCD1LG2 active protein

Background: Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.

Product Categories/Family for PDCD1LG2 active protein

Cancer Drug Targets

References

Latchman Y, et al. (2001) PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2: 261-8.Carreno BM, et al. (2005) Therapeutic opportunities in the B7/CD28 family of ligands and receptors. Curr Opin Pharmacol. 5(4): 424-30.Radhakrishnan S, et al. (2007) B7-DC/PD-L2 cross-linking induces NF-kappaB-dependent protection of dendritic cells from cell death. J Immunol. 178(3): 1426-32.

NCBI and Uniprot Product Information

• NCBI GI #: 190014605
• NCBI GeneID: 80380
• NCBI Accession #: NP_079515.2
• NCBI GenBank Nucleotide #: NM_025239.3
• UniProt Accession #: Q9BQ51; Q14CN8; Q5T7Z6; Q6JXL8; Q6JXL9
• Molecular Weight: 20,745 Da
• NCBI Official Full Name: programmed cell death 1 ligand 2
• NCBI Official Synonym Full Names: programmed cell death 1 ligand 2
• NCBI Official Symbol: PDCD1LG2
• NCBI Official Synonym Symbols: B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
• NCBI Protein Information: programmed cell death 1 ligand 2
• UniProt Protein Name: Programmed cell death 1 ligand 2
• Protein Family: Programmed cell death
• UniProt Gene Name: PDCD1LG2
• UniProt Synonym Gene Names: B7DC; CD273; PDCD1L2; PDL2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; B7-DC
• UniProt Entry Name: PD1L2_HUMAN

Uniprot Description

PDCD1LG2: Involved in the costimulatory signal, essential for T- cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 9p24.2

Cellular Component: plasma membrane

Biological Process: negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; negative regulation of interleukin-10 production; T cell costimulation