Rabbit anti-Human CD247 Polyclonal Antibody | anti-CD247 antibody

CD247 Antibody

Cat. Num. AAA7049563

• Gene Names: CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: >95%, Protein G purified
• Synonyms: CD247; Polyclonal Antibody; CD247 Antibody; T-cell surface glycoprotein CD3 zeta chain (T-cell receptor T3 zeta chain) (CD antigen CD247); CD3Z T3Z TCRZ; anti-CD247 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid

• Applicable Applications for anti-CD247 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
• Immunogen: Recombinant Human T-cell surface glycoprotein CD3 zeta chain protein (88-153AA)
• Conjugate: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Species: Human
• Research Area: Immunology
• Target Names: CD247
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-1124 / sc-20919
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Jurkat whole cell lysateAll lanes: CD247 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 19 KDaObserved band size: 19 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lymph node tissue using MBS7049563 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS7049563 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of HepG2 cells using MBS7049563 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunoprecipitation (IP)

(Immunoprecipitating CD247 in jurkat whole cell lysateLane 1: Rabbit control IgG instead of MBS7049563 in jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7049563 (8ug) + jurkat whole cell lysate (500ug)Lane 3: Jurkat whole cell lysate (10ug))

Related Product Information for anti-CD247 antibody

Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.

NCBI and Uniprot Product Information

• NCBI GI #: 4557431
• NCBI GeneID: 919
• NCBI Accession #: NP_000725.1
• NCBI GenBank Nucleotide #: NM_000734.3
• UniProt Accession #: P20963; Q5VX13; Q8TAX4; B1AK49; A46461
• NCBI Official Full Name: T-cell surface glycoprotein CD3 zeta chain isoform 2
• NCBI Official Synonym Full Names: CD247 molecule
• NCBI Official Symbol: CD247
• NCBI Official Synonym Symbols: T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
• NCBI Protein Information: T-cell surface glycoprotein CD3 zeta chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 zeta chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD247
• UniProt Synonym Gene Names: CD3Z; T3Z; TCRZ
• UniProt Entry Name: CD3Z_HUMAN

NCBI Description

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3Z: a T cell surface glycoprotein that is a component of the T cell antigen receptor. Plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the CD3-epsilon results in impaired immune response. Two alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: cytoplasm; plasma membrane; T cell receptor complex

Molecular Function: identical protein binding; protein binding

Biological Process: regulation of defense response to virus by virus; regulation of immune response; T cell costimulation; T cell receptor signaling pathway

Disease: Immunodeficiency 25