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Western Blot (WB) (Western blot analysis of extracts from COS7  , using Phospho-CD226/DNAM-1 (Ser329) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Rabbit CD226/DNAM-1 Polyclonal Antibody | anti-CD226/DNAM-1 antibody

Phospho-CD226/DNAM-1 (Ser329) Antibody

Gene Names
CD226; PTA1; DNAM1; DNAM-1; TLiSA1
Reactivity
Human, Mouse, Monkey
Predicted Reactivity: Pig (100%), Horse (100%)
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
CD226/DNAM-1; Polyclonal Antibody; Phospho-CD226/DNAM-1 (Ser329) Antibody; adhesion glycoprotein; CD226; CD226 antigen; CD226 molecule; CD226_HUMAN; DNAM 1; DNAM-1; DNAM1; DNAX accessory molecule 1; platelet and T cell activation antigen 1; PTA1; T lineage specific activation antigen 1 antigen; TLiSA1; anti-CD226/DNAM-1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Monkey
Predicted Reactivity: Pig (100%), Horse (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-CD226/DNAM-1 (Ser329) Antibody detects endogenous levels of CD226/DNAM-1 only when phosphorylated at Ser329.
Tissue Specificity: Expressed by peripheral blood T-lymphocytes.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD226/DNAM-1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human CD226/DNAM-1 around the phosphorylation site of Ser329.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Interacts with PVR and NECTIN2. Competes with PVRIG for NECTIN2-binding.
Subcellular Location
Cell membrane>Single-pass type I membrane protein.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from COS7  , using Phospho-CD226/DNAM-1 (Ser329) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from COS7  , using Phospho-CD226/DNAM-1 (Ser329) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Immunohistochemistry (IHC)

(Staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC) (Staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-CD226/DNAM-1 antibody
Involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell. Cell surface receptor for NECTIN2. Upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Competes with PVRIG for NECTIN2-binding.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,614 Da
NCBI Official Full Name
CD226 antigen
NCBI Official Synonym Full Names
CD226 molecule
NCBI Official Symbol
CD226
NCBI Official Synonym Symbols
PTA1; DNAM1; DNAM-1; TLiSA1
NCBI Protein Information
CD226 antigen; adhesion glycoprotein; DNAX accessory molecule 1; DNAX accessory molecule-1; platelet and T cell activation antigen 1; T lineage-specific activation antigen 1 antigen
UniProt Protein Name
CD226 antigen
UniProt Gene Name
CD226
UniProt Synonym Gene Names
DNAM1; DNAM-1
UniProt Entry Name
CD226_HUMAN

NCBI Description

This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. [provided by RefSeq, Jul 2008]

Uniprot Description

DNAM-1: Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.

Protein type: Cell adhesion; Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q22.3

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; external side of plasma membrane; lipid raft

Molecular Function: integrin binding; protein binding; cell adhesion molecule binding; protein kinase binding

Biological Process: regulation of immune response; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of immunoglobulin mediated immune response; cytokine production; cell recognition; cell adhesion; positive regulation of natural killer cell mediated cytotoxicity; signal transduction; positive regulation of mast cell activation

Research Articles on CD226/DNAM-1

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Product Notes

The CD226/DNAM-1 cd226 (Catalog #AAA9613278) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-CD226/DNAM-1 (Ser329) Antibody reacts with Human, Mouse, Monkey Predicted Reactivity: Pig (100%), Horse (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's CD226/DNAM-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the CD226/DNAM-1 cd226 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD226/DNAM-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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