Rabbit anti-Human CD20 Polyclonal Antibody | anti-CD20 antibody

Rabbit Polyclonal to Human CD20

Cat. Num. AAA242125

• Gene Names: MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation
• Purity: Immunoaffinity Purified
• Synonyms: CD20; Polyclonal Antibody; Rabbit Polyclonal to Human CD20; Anti-CD20 Antibody (C-Terminus) IHC-plus; MS4A1; B-lymphocyte antigen CD20; B1; Bp35; CD20 antigen; CD20 receptor; LEU-16; CVID5; Human CD20; anti-CD20 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes CD20. Species cross-reactivity: Human.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, pH 7.6, 1% BSA, 0.1% sodium azide
• Concentration: 0.2 mg/ml (varies by lot)
• Sequence Length: 297

• Applicable Applications for anti-CD20 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IHC-P (1:100), IP, WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide derived from C-terminus of human CD20 protein.
• Immunogen Type: Synthetic peptide
• Immunogen: CD20 antibody was raised against synthetic peptide derived from C-terminus of human CD20 protein.
• Antigen Modification: C-Terminus
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-MS4A1 / CD20 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Related Product Information for anti-CD20 antibody

CD20 is also called L26 or membrane spanning 4 domains (MS4A1). It is a 33 kd phosphoprotein with 3 hydrophobic regions that traverse the cell membrane, creating a structure similar to an ion channel that allows for the influx of calcium ions required for cell activation. Initially expressed on B cells after CD19/CD10 expression and before CD21/CD22 and surface immunoglobulin expression. It is retained on mature B cells until plasma cell development.

NCBI and Uniprot Product Information

• NCBI GI #: 23110989
• NCBI GeneID: 931
• NCBI Accession #: NP_690605.1
• NCBI GenBank Nucleotide #: NM_152866.2
• UniProt Accession #: P11836; P08984; Q13963; A6NMS4; B4DT24
• Molecular Weight: 14,581 Da
• NCBI Official Full Name: B-lymphocyte antigen CD20
• NCBI Official Synonym Full Names: membrane-spanning 4-domains, subfamily A, member 1
• NCBI Official Symbol: MS4A1
• NCBI Official Synonym Symbols: B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• NCBI Protein Information: B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1
• UniProt Protein Name: B-lymphocyte antigen CD20
• Protein Family: CD209 antigen
• UniProt Gene Name: MS4A1
• UniProt Synonym Gene Names: CD20
• UniProt Entry Name: CD20_HUMAN

NCBI Description

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane

Molecular Function: epidermal growth factor receptor binding

Biological Process: B cell proliferation; humoral immune response

Disease: Immunodeficiency, Common Variable, 5