Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using CD2 Tail-binding Antibody.)
Rabbit anti-Human, Mouse CD2 Tail-binding Polyclonal Antibody | anti-CD2BP2 antibody
CD2 Tail-binding Antibody
Gene Names
CD2BP2; LIN1; Snu40; FWP010; U5-52K; PPP1R59
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CD2 Tail-binding; Polyclonal Antibody; CD2 Tail-binding Antibody; CD2 (cytoplasmic tail) binding protein 2; CD2 antigen (cytoplasmic tail) binding protein 2; CD2 antigen cytoplasmic tail-binding protein 2; CD2 cytoplasmic domain-binding protein 2; CD2 tail-binding protein 2; CD2B2_HUMAN; Cd2bp2; FWP010; LIN1; Snu40; U5 snRNP 52K protein; U5-52K; anti-CD2BP2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CD2 Tail-binding antibody detects endogenous levels of total CD2 Tail-binding
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
341
Applicable Applications for anti-CD2BP2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human CD2 Tail-binding
Subcellular Location
Cytoplasm. Nucleus. Predominantly Nuclear.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (91%), Bovine (91%), Horse (91%), Sheep (91%), Rabbit (91%), Dog (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using CD2 Tail-binding Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using CD2 Tail-binding Antibody.)
Western Blot (WB)
(Western blot analysis of CD2 Tail-binding using A549 whole cell lysates)
Western Blot (WB)
(Western blot analysis of CD2 Tail-binding using A549 whole cell lysates)
Immunofluorescence (IF)
(MBS9608961 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9608961 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-CD2BP2 antibody
Function: Involved in pre-mRNA splicing as component of the U5 snRNP complex that is involved in spliceosome assembly.
Subunit Structure: Component of the U5 snRNP complex composed of the U5 snRNA and at least PRPF6, PRPF8, SNRNP200, EFTUD2, SNRNP40, DDX23, TXNL4A and CD2BP2. Interacts directly with TXNL4A and PRPF6. Interacts (via GYF domain) with CD2 (via Pro-rich sequence in the cytoplasmic domain). Interacts with PQBP1.
Subunit Structure: Component of the U5 snRNP complex composed of the U5 snRNA and at least PRPF6, PRPF8, SNRNP200, EFTUD2, SNRNP40, DDX23, TXNL4A and CD2BP2. Interacts directly with TXNL4A and PRPF6. Interacts (via GYF domain) with CD2 (via Pro-rich sequence in the cytoplasmic domain). Interacts with PQBP1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 37 kDa
Predicted: 38 kDa
Predicted: 38 kDa
NCBI Official Full Name
CD2 antigen cytoplasmic tail-binding protein 2
NCBI Official Synonym Full Names
CD2 cytoplasmic tail binding protein 2
NCBI Official Symbol
CD2BP2
NCBI Official Synonym Symbols
LIN1; Snu40; FWP010; U5-52K; PPP1R59
NCBI Protein Information
CD2 antigen cytoplasmic tail-binding protein 2
UniProt Protein Name
CD2 antigen cytoplasmic tail-binding protein 2
Protein Family
UniProt Gene Name
CD2BP2
UniProt Synonym Gene Names
KIAA1178; CD2 cytoplasmic domain-binding protein 2; CD2 tail-binding protein 2; U5-52K
NCBI Description
This gene encodes a bi-functional protein. In the cytoplasm, the encoded protein binds the cytoplasmic tail of human surface antigen CD2 via its C-terminal GYF domain, and regulate CD2-triggered T lymphocyte activation. In the nucleus, this protein is a component of the U5 small nuclear ribonucleoprotein complex and is involved in RNA splicing. A pseudogene has been identified on chromosome 7. Alternative splicing results in multiple transcript variants but their biological validity has not been determined. [provided by RefSeq, Nov 2008]
Uniprot Description
Involved in pre-mRNA splicing as component of the U5 snRNP complex that is involved in spliceosome assembly.
Research Articles on CD2BP2
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Product Notes
The CD2BP2 cd2bp2 (Catalog #AAA9608961) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD2 Tail-binding Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD2 Tail-binding can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000-3000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CD2BP2 cd2bp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD2 Tail-binding, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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