Western Blot (WB)
(Western blot analysis on F9 (A) and NCCIT (B) cell lysates using anti-CD19 rabbit polyclonal antibody.)
anti-Human, Mouse CD19 Polyclonal Antibody | anti-CD19 antibody
CD19 Antibody
Gene Names
CD19; B4; CVID3
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purified
Synonyms
CD19; Polyclonal Antibody; CD19 Antibody; anti-CD19 antibody
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of CD19.
Purity/Purification
Affinity Purified
Form/Format
Liquid
Sequence Length
557
Applicable Applications for anti-CD19 antibody
Western Blot (WB)
Application Notes
WB: 1:2,000-1:5,000
Cellular Localization
Cell Membrane
Positive Control
F9, NCCIT
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis on F9 (A) and NCCIT (B) cell lysates using anti-CD19 rabbit polyclonal antibody.)
Western Blot (WB)
(Western blot analysis on F9 (A) and NCCIT (B) cell lysates using anti-CD19 rabbit polyclonal antibody.)
Related Product Information for anti-CD19 antibody
B-lymphocyte antigen CD19 also known as CD19 (Cluster of Differentiation 19), is a 95 kDa type-I transmembrane glycoprotein which is restricted to B cell antigen. On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function. CD19 is also found on the follicular dendritic cells and the early cells of myelomonocytic lineage but not on normal T cells, NK cells, monocytes, granulocytes, erythrocytes and platelets.
References
1. "An antibody-deficiency syndrome due to mutations in the CD19 gene."van Zelm M.C., Reisli I., van der Burg M., Castano D., van Noesel C.J.M., van Tol M.J.D., Woellner C., Grimbacher B., Patino P.J., van Dongen J.J.M., Franco J.L.N. Engl. J. Med. 354:1901-1912(2006). 2. "Systematic analysis of the role of CD19 cytoplasmic tyrosines in enhancement of activation in Daudi human B cells: clustering of phospholipase C and Vav and of Grb2 and Sos with different CD19 tyrosines."Brooks S.R., Li X., Volanakis E.J., Carter R.H.J. Immunol. 164:3123-3131(2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80-95kDa
NCBI Official Full Name
B-lymphocyte antigen CD19 isoform 1
NCBI Official Synonym Full Names
CD19 molecule
NCBI Official Symbol
CD19
NCBI Official Synonym Symbols
B4; CVID3
NCBI Protein Information
B-lymphocyte antigen CD19
UniProt Protein Name
B-lymphocyte antigen CD19
Protein Family
UniProt Gene Name
CD19
NCBI Description
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
Uniprot Description
Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Research Articles on CD19
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Product Notes
The CD19 cd19 (Catalog #AAA8527293) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The CD19 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD19 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:2,000-1:5,000. Researchers should empirically determine the suitability of the CD19 cd19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD19, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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