Rabbit anti-Human CD163 Polyclonal Antibody | anti-CD163 antibody

CD163, NT (CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163, Soluble CD163) (MaxLight 405)

Cat. Num. AAA6282811

• Gene Names: CD163; M130; MM130
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: CD163; Polyclonal Antibody; NT (CD163; M130; Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor; Soluble CD163) (MaxLight 405); Soluble CD163; anti-CD163 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CD163.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-CD163 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 245-274 in the N-terminal region of human CD163, UniProt Accession #Q86VB7.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6007064. CD163 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry in formalin-fixed and paraffin-embedded human hepatocarcinoma usingMBS6007064, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6007064 for immunohistochemistry.)

Product Categories/Family for anti-CD163 antibody

Antibodies; Receptors; Scavenger

References

1. Buehler, P.W., Abraham, B. Blood 113 (11), 2578-2586 (2009). 2. Fabriek, B.O., van Bruggen, R. Blood 113 (4), 887-892 (2009). 3. Strauss, M. and Levy, A.P. Mol. Cell. Biochem. 317 (1-2), 131-135 (2008). 4. Groselj-Grenc, M., Ihan, A. Mediators Inflamm. 2008, 202646 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 344179110
• NCBI GeneID: 9332
• NCBI Accession #: NP_004235.4
• NCBI GenBank Nucleotide #: NM_004244.5
• UniProt Accession #: Q86VB7; Q07898; Q07899; Q07900; Q07901; Q2VLH7; C9JIG2
• Molecular Weight: 125,451 Da
• NCBI Official Full Name: scavenger receptor cysteine-rich type 1 protein M130 isoform a
• NCBI Official Synonym Full Names: CD163 molecule
• NCBI Official Symbol: CD163
• NCBI Official Synonym Symbols: M130; MM130
• NCBI Protein Information: scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen
• UniProt Protein Name: Scavenger receptor cysteine-rich type 1 protein M130
• Protein Family: Scavenger receptor cysteine-rich type 1 protein
• UniProt Gene Name: CD163
• UniProt Synonym Gene Names: M130; sCD163
• UniProt Entry Name: C163A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: protein binding; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; acute-phase response