KPNA2 recombinant protein

KPNA2, 1-529aa, Human, His tag, E Coli

Cat. Num. AAA204676

• Gene Names: KPNA2; QIP2; RCH1; IPOA1; SRP1alpha; SRP1-alpha
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: KPNA2; 1-529aa; Human; His tag; E Coli; Importin subunit alpha-2; IPOA1; QIP2; SRP1alpha; KPNA2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 1mM DTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSTNEN ANTPAARLHR FKNKGKDSTE MRRRRIEVNV ELRKAKKDDQ MLKRRNVSSF PDDATSPLQE NRNNQGTVNW SVDDIVKGIN SSNVENQLQA TQAARKLLSR EKQPPIDNII RAGLIPKFVS FLGRTDCSPI QFESAWALTN IASGTSEQTK VVVDGGAIPA FISLLASPHA HISEQAVWAL GNIAGDGSVF RDLVIKYGAV DPLLALLAVP DMSSLACGYL RNLTWTLSNL CRNKNPAPPI DAVEQILPTL VRLLHHDDPE VLADTCWAIS YLTDGPNERI GMVVKTGVVP QLVKLLGASE LPIVTPALRA IGNIVTGTDE QTQVVIDAGA LAVFPSLLTN PKTNIQKEAT WTMSNITAGR QDQIQQVVNH GLVPFLVSVL SKADFKTQKE AVWAVTNYTS GGTVEQIVYL VHCGIIEPLM NLLTAKDTKI ILVILDAISN IFQAAENLGE TEKLSIMIEE CGGLDKIEAL QNHENESVYK ASLSLIEKYF SVEEEEDQNV VPETTSEGYT FQVQDGAPGT FNF
• Sequence Length: 529

• Applicable Applications for KPNA2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for KPNA2 recombinant protein

Karyopherin alpha2 (KPNA2) is a member of the karyopherin alpha family. This protein plays a key role in the nuclear import of proteins with a classical nuclear localization signal (NLS). KPNA2 bound in vitro specifically and directly to substrates containing either a simple or bipartite NLS motif. It promoted docking of import substrates to the nuclear envelope and together with recombinant human Ran reconstituted complete nuclear protein import. Recombinant human KPNA2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for KPNA2 recombinant protein

Metabolism

References

Moroianu J., et al. (1995) Proc. Natl. Acad. Sci. u.S.A. 92:6532-6536; Braem C.V., et al. (2002) J. Biol. Chem. 277:19673-19678

NCBI and Uniprot Product Information

• NCBI GI #: 13543657
• NCBI GeneID: 3838
• Molecular Weight: 60.5 kDa (553aa)
• NCBI Official Full Name: Karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
• NCBI Official Synonym Full Names: karyopherin subunit alpha 2
• NCBI Official Symbol: KPNA2
• NCBI Official Synonym Symbols: QIP2; RCH1; IPOA1; SRP1alpha; SRP1-alpha
• NCBI Protein Information: importin subunit alpha-1
• UniProt Protein Name: Importin subunit alpha-1
• UniProt Gene Name: KPNA2
• UniProt Synonym Gene Names: RCH1; SRP1

NCBI Description

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.