Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prominin-1 (PROM1) Standard Curve.)
Mouse Prominin-1 (PROM1) ELISA Kit | PROM1 elisa kit
Mouse Prominin-1 (PROM1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prominin-1 (PROM1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prominin-1 (PROM1) Standard Curve.)
Background: CD133 is a glycoprotein also known in humans and rodents as Prominin 1 (PROM1).It is the founding member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localizes to cellular protrusions. CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastomas, neuronal and glial stem cells and some other cell types. In contrast, AC133-1 was prominent in fetal brain and adult skeletal muscle and absent in fetal liver and kidney and in adult pancreas, kidney, bone marrow, hematopoietic stem cells, and placenta. RT-PCR and flow cytometric analysis revealed expression of AC133-2 in basal epidermal stem cells, and the expression was downregulated in cultured basal cells.
NCBI and Uniprot Product Information
Uniprot Description
CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Cell surface; Membrane protein, integral
Cellular Component: extracellular space; microvillus; cell surface; integral to plasma membrane; endoplasmic reticulum; ER-Golgi intermediate compartment; integral to membrane; cilium; stereocilium; photoreceptor outer segment; cell projection; membrane; apical plasma membrane; plasma membrane; brush border; vesicle
Molecular Function: actinin binding; cadherin binding
Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye
Research Articles on PROM1
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Product Notes
The Mouse PROM1 prom1 (Catalog #AAA9717257) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717257 ELISA Kit recognizes Mouse PROM1. It is sometimes possible for the material contained within the vial of "Prominin-1 (PROM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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