Rabbit anti-Rat, Mouse CD13 Polyclonal Antibody | anti-ANPEP antibody

Anti-CD13 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101650

• Gene Names: Anpep; Apm; Apn; KZP; AP-M; AP-N; Lap1; rAPN
• Reactivity: Rat, Mouse
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: CD13; Polyclonal Antibody; Anti-CD13 Antibody; Rabbit Polyclonal; CD13/ANPEP Antibody; Rabbit PAb; Antigen Affinity Purified; alanyl (membrane) aminopeptidase; anti-ANPEP antibody

• Host: Rabbit
• Reactivity: Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat CD13
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ANPEP antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Rat CD13/ANPEP
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Rat CD13/ANPEP, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse Anpep in mouse intestine with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse Anpep in mouse kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse Anpep in rat intestine with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse Anpep in rat kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-ANPEP antibody

Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.

References

Pasqualini R, et al. (2000) Aminopeptidase N is a receptor for tumor-homing peptides and a target for inhibiting angiogenesis.Cancer Res. 60(3): 722-7.Mabjeesh SJ, et al. (2005) Aminopeptidase N gene expression and abundance in caprine mammary gland is influenced by circulating plasma peptide. J Dairy Sci. 88(6): 2055-64.Dunkel B, et al. (2009) Neutrophil and platelet activation in equine recurrent airway obstruction is associated with increased neutrophil CD13 expression, but not platelet CD41/61 and CD62P or neutrophil-platelet aggregate formation. Vet Immunol Immunopathol. 131(1-2): 25-32.

NCBI and Uniprot Product Information

• NCBI GI #: 13591914
• NCBI GeneID: 81641
• NCBI Accession #: NP_112274.1
• NCBI GenBank Nucleotide #: NM_031012.1
• UniProt Accession #: P15684; Q9JHP4
• Molecular Weight: 109,449 Da
• NCBI Official Full Name: aminopeptidase N
• NCBI Official Synonym Full Names: alanyl aminopeptidase, membrane
• NCBI Official Symbol: Anpep
• NCBI Official Synonym Symbols: Apm; Apn; KZP; AP-M; AP-N; Lap1; rAPN
• NCBI Protein Information: aminopeptidase N
• UniProt Protein Name: Aminopeptidase N
• Protein Family: Aminopeptidase
• UniProt Gene Name: Anpep
• UniProt Synonym Gene Names: AP-N; rAPN; AP-M; KZP
• UniProt Entry Name: AMPN_RAT

NCBI Description

a cell surface Zinc-peptidase [RGD, Feb 2006]

Uniprot Description

CD13: B

Protein type: EC 3.4.11.2; Membrane protein, integral; Protease; Other Amino Acids Metabolism - glutathione; Receptor, misc.

Cellular Component: cytoplasm; ER-Golgi intermediate compartment; external side of plasma membrane; extracellular space; integral to membrane; lysosomal membrane; plasma membrane; vesicle membrane

Molecular Function: aminopeptidase activity; metallopeptidase activity; peptide binding; zinc ion binding

Biological Process: angiogenesis; aromatic compound metabolic process; cell differentiation; peptide catabolic process; proteolysis