Western Blot (WB)
(Anti-CCR5 antibody, MBS175709, Western blottingLane 1: JURKAT Cell LysateLane 2: COLO320 Cell Lysate )
Rabbit CCR5 Polyclonal Antibody | anti-CCR5 antibody
Anti-CCR5 antibody
Predicted to work with: Rat
Predicted to work with: Rat
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Predicted Species: Ms, Ratt
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM, pH6.0, for 20 mins is required for the staining of formalin/pafaffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Western Blot (WB)
(Anti-CCR5 antibody, MBS175709, Western blottingLane 1: JURKAT Cell LysateLane 2: COLO320 Cell Lysate )
Western Blot (WB)
(Anti-CCR5 antibody, MBS175709, Western blottingLane 1: JURKAT Cell LysateLane 2: COLO320 Cell Lysate )
Immunohistochemistry (IHC)
(Anti-CCR5 antibody, MBS175709, IHC(P)IHC(P): Human Rectal Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-CCR5 antibody, MBS175709, IHC(P)IHC(P): Human Rectal Cancer Tissue )
Western Blot (WB)
(Anti-CCR5 antibody, MBS175709, IHC(P)All lanes: Anti CCR5 (MBS175709) at 0.5ug/mlWB: Mouse Liver Tissue Lysate at 50ugPredicted bind size: 40KDObserved bind size: 40KD )
Western Blot (WB)
(Anti-CCR5 antibody, MBS175709, IHC(P)All lanes: Anti CCR5 (MBS175709) at 0.5ug/mlWB: Mouse Liver Tissue Lysate at 50ugPredicted bind size: 40KDObserved bind size: 40KD )
Background: Human CC chemokine receptor 5(CCR5), which is 17.5 kb from the CMKBR2 gene, encodes a 352-amino acid protein with a calculated molecular mass of 40,600 Da. It is organized into four exons and two introns. Human CCR5 is a co-receptor for macrophage-tropic strains of human immunodeficiency virus(HIV)-1 and is expressed by bone-marrow-derived cells. CCR5 is expressed on neurons, astrocytes, and microglia in the central nervous system, and on epithelium, endothelium, vascular smooth muscle and fibroblasts in other tissues. Functionally, CCR5 mediates the activation of cells by the chemokines macrophage inflammatory protein-1alpha, macrophage inflammatory protein-1beta, and RANTES, and serves as a fusion cofactor for macrophage-tropic strains of human immunodeficiency virus type 1.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
CCR5: a 7-transmembrane G-linked receptor for a number of inflammatory C-C type chemokines including MIP-1-alpha, MIP-1-beta and RANTES. Transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (along with CD4) for HIV-1 R5 isolates. Interacts with PRAF2. Interacts with HIV-1 surface protein gp120. Efficient ligand binding to CCL3/MIP-1alpha and CCR4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications. Glycosylation on S6 is required for efficient binding of CCL4. Interacts with ADRBK1. Interacts with ARRB1 and ARRB2. Variations in CCR5 are associated with resistance or susceptibility to immunodeficiency virus type 1 (resistance or susceptibility to HIV-1). Variations in CCR5 gene also influence the rate of progression to AIDS after infection. R60S variant, a naturally occurring mutation in a conserved residue in the first intracellular domain of CCR5, results in reduced amounts of the protein in the membrane and consequently may be associated with reduced susceptibility to infection by microbes that depend on these molecules as their receptors. Variations in CCR5 are associated with susceptibility to West Nile virus (WNV) infection
Protein type: Receptor, GPCR; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, cytokine; Membrane protein, multi-pass; GPCR, family 1
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: cell surface; integral to plasma membrane; cytoplasm; plasma membrane; endosome; external side of plasma membrane
Molecular Function: protein binding; C-C chemokine receptor activity; chemokine receptor activity; coreceptor activity; C-C chemokine binding; actin binding; phosphoinositide phospholipase C activity
Biological Process: viral reproduction; MAPKKK cascade; calcium-mediated signaling; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; chemotaxis; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; calcium ion transport; dendritic cell chemotaxis; cellular defense response; immune response; entry into host cell; inflammatory response
Disease: West Nile Virus, Susceptibility To; Diabetes Mellitus, Insulin-dependent, 22; Hepatitis C Virus, Susceptibility To
Research Articles on CCR5
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Product Notes
The CCR5 ccr5 (Catalog #AAA175709) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CCR5 antibody reacts with Reacts with: Human, Mouse Predicted to work with: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CCR5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Hu Predicted Species: Ms, Ratt Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM, pH6.0, for 20 mins is required for the staining of formalin/pafaffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the CCR5 ccr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCR5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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