Rat Pro-amylin (IAPP) ELISA Kit | IAPP elisa kit
Rat Pro-amylin (IAPP) ELISA Kit
Reactivity
Rat
Synonyms
Pro-amylin (IAPP); Rat Pro-amylin (IAPP) ELISA Kit; IAPP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of ProIAPP. No significant cross-reactivity or interference between ProIAPP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ProIAPP and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for IAPP elisa kit
Intended Uses: This ProIAPP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ProIAPP. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: ProIAPP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ProIAPP antibody and an ProIAPP-HRP conjugate. The assay sample and buffer are incubated together with ProIAPP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ProIAPP concentration since ProIAPP from samples and ProIAPP-HRP conjugate compete for the anti-ProIAPP antibody binding site. Since the number of sites is limited, as more sites are occupied by ProIAPP from the sample, fewer sites are left to bind ProIAPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ProIAPP concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ProIAPP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ProIAPP antibody and an ProIAPP-HRP conjugate. The assay sample and buffer are incubated together with ProIAPP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ProIAPP concentration since ProIAPP from samples and ProIAPP-HRP conjugate compete for the anti-ProIAPP antibody binding site. Since the number of sites is limited, as more sites are occupied by ProIAPP from the sample, fewer sites are left to bind ProIAPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ProIAPP concentration in each sample is interpolated from this standard curve.
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