Rabbit anti-Human CCNG1 Polyclonal Antibody | anti-CCNG1 antibody

CCNG1, NT (CCNG1, CCNG, CYCG1, Cyclin-G1) (Biotin)

Cat. Num. AAA6282412

• Gene Names: CCNG1; CCNG
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CCNG1; Polyclonal Antibody; NT (CCNG1; CCNG; CYCG1; Cyclin-G1) (Biotin); Cyclin-G1; anti-CCNG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CCNG1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CCNG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-53 amino acids from the N-terminal region of human CCNG1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CCNG1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CCNG1 antibody detected the CCNG1 protein (arrow).)

Flow Cytometry (FC/FACS)

(CCNG1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CCNG1 antibody

The eukaryotic cell cycle is governed by cyclin-dependent protein kinases (CDKs) whose activities are regulated by cyclins and CDK inhibitors. The protein encoded by this gene is a member of the cyclin family and contains the cyclin box. The encoded protein lacks the protein destabilizing (PEST) sequence that is present in other family members. Transcriptional activation of this gene can be induced by tumor protein p53. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq].

Product Categories/Family for anti-CCNG1 antibody

Antibodies; Cyclin

References

Cunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009). Fornari, F., et al. Cancer Res. 69(14):5761-5767(2009). Li, H., et al. Mol. Cell. Biol. 29(3):919-928(2009). Piscopo, D.M., et al. Cancer Res. 68(14):5581-5590(2008). Seo, H.R., et al. J. Biol. Chem. 283(23):15601-15610(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4757934
• NCBI GeneID: 900
• NCBI Accession #: NP_004051.1; NP_954854.1
• NCBI GenBank Nucleotide #: NM_004060.3
• UniProt Accession #: P51959; Q15757; Q96L32; B2R7B2; D3DQK7
• Molecular Weight: 34,074 Da
• NCBI Official Full Name: cyclin-G1
• NCBI Official Synonym Full Names: cyclin G1
• NCBI Official Symbol: CCNG1
• NCBI Official Synonym Symbols: CCNG
• NCBI Protein Information: cyclin-G1
• UniProt Protein Name: Cyclin-G1
• Protein Family: Cyclin
• UniProt Gene Name: CCNG1
• UniProt Synonym Gene Names: CCNG; CYCG1; Cyclin-G
• UniProt Entry Name: CCNG1_HUMAN

NCBI Description

The eukaryotic cell cycle is governed by cyclin-dependent protein kinases (CDKs) whose activities are regulated by cyclins and CDK inhibitors. The protein encoded by this gene is a member of the cyclin family and contains the cyclin box. The encoded protein lacks the protein destabilizing (PEST) sequence that is present in other family members. Transcriptional activation of this gene can be induced by tumor protein p53. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNG1: May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation. Belongs to the cyclin family. Cyclin G subfamily.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q32-q34

Cellular Component: cell soma; perinuclear region of cytoplasm; dendrite; nucleus

Molecular Function: protein domain specific binding; protein binding

Biological Process: mitosis; cell division; mitotic cell cycle G2/M transition DNA damage checkpoint; regulation of cyclin-dependent protein kinase activity; brain development; response to organic nitrogen; cell growth; syncytium formation; negative regulation of apoptosis