Rabbit anti-Rhesus CCL21 Polyclonal Antibody | anti-CCL21 antibody

Anti-CCL21 Antibody, Rabbit Polyclonal

Cat. Num. AAA8113213

• Reactivity: Rhesus
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: CCL21; Polyclonal Antibody; Anti-CCL21 Antibody; Rabbit Polyclonal; CCL21/6Ckine Antibody; Rabbit PAb; Antigen Affinity Purified; chemokine (C-C motif) ligand 21; anti-CCL21 antibody

• Host: Rabbit
• Reactivity: Rhesus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rhesus CCL21/SCYA21
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CCL21 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Immunogen: Recombinant Rhesus CCL21/SCYA21 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rhesus CCL21/SCYA21. CCL21/SCYA21 specific IgG was purified by Rhesus CCL21/SCYA21 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CCL21 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 60 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-CCL21 antibody

Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 21(CCL21), also known as 6Ckine, exodus-2, and secondary lymphoid-tissue chemokine(SLC), is a small cytokine belonging to the C-C chemokine family. CCL21 takes its name 6Ckine for its consititutively six conserved cysteine residues but not four cysteines typical to chemokines. CCL21 has function in ininducing vigorous calcium migrations and chemotactic responses.

Product Categories/Family for anti-CCL21 antibody

Neuroinflammation

References

Laing KJ, et al. (2004) Chemokines. Developmental and comparative immunology. 28(5): 443-60.
Cocchi F, et al. (1995) Identification of RANTES, MIP-1a, and MIP-1b as the major HIV-suppressive factor produced by CD8+ T cells. Science. 270 (5243): 1811-5.
Yoshida R, et al. (1998) Secondary lymphoid-tissue chemokine is a functional ligand for the CC chemokine receptor CCR7. The journal of biological chemistry. 273: 7118-22.

NCBI and Uniprot Product Information

• NCBI GI #: 74136271
• NCBI GeneID: 574183
• NCBI Accession #: NP_001028027.1
• NCBI GenBank Nucleotide #: NM_001032855.1
• UniProt Accession #: Q8HYP5
• Molecular Weight: 14,368 Da
• NCBI Official Full Name: C-C motif chemokine 21
• NCBI Official Symbol: CCL21
• NCBI Protein Information: C-C motif chemokine 21; chemokine CCL21/6CKINE; small-inducible cytokine A21
• UniProt Protein Name: C-C motif chemokine 21
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL21
• UniProt Entry Name: CCL21_MACMU