CCL21 active protein

CCL21 Protein, Human, Recombinant

Cat. Num. AAA8120300

• Gene Names: CCL21; ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21
• Purity: >98% as determined by SDS-PAGE
• Synonyms: CCL21; CCL21 Protein; Human; Recombinant; Human CCL21/6Ckine Protein; 6Ckine Protein; CKb9 Protein; ECL Protein; MGC34555 Protein; RP11-195F19.22-001 Protein; SCYA21 Protein; SLC Protein; TCA4 Protein; chemokine (C-C motif) ligand 21; CCL21 active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 40mM Tris, 0.3 M NaCl, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro134

• Species: Human
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human CCL21 at 2ug/ml (100uL/well) can bind human IGFBP7 with a linear range of 0.16-4ug/ml.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 24
• Tag: Native
• Protein Construction: A DNA sequence encoding the human CCL21 (O00585)(Met 1-Pro 134) was expressed and purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CCL21 active protein

Background: Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 21(CCL21), also known as 6Ckine, exodus-2, and secondary lymphoid-tissue chemokine(SLC), is a small cytokine belonging to the C-C chemokine family. CCL21 takes its name 6Ckine for its consititutively six conserved cysteine residues but not four cysteines typical to chemokines. CCL21 has function in ininducing vigorous calcium migrations and chemotactic responses.

Product Categories/Family for CCL21 active protein

Neuroinflammation

References

Laing KJ, et al. (2004) Chemokines. Developmental and comparative immunology. 28(5): 443-60.
Cocchi F, et al. (1995) Identification of RANTES, MIP-1a, and MIP-1b as the major HIV-suppressive factor produced by CD8+ T cells. Science. 270 (5243): 1811-5.
Yoshida R, et al. (1998) Secondary lymphoid-tissue chemokine is a functional ligand for the CC chemokine receptor CCR7. The journal of biological chemistry. 273: 7118-22.

NCBI and Uniprot Product Information

• NCBI GI #: 4128129
• NCBI GeneID: 6366
• UniProt Accession #: O00585
• Molecular Weight: 14,646 Da
• NCBI Official Full Name: secondary lymphoid tissue chemokine
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 21
• NCBI Official Symbol: CCL21
• NCBI Official Synonym Symbols: ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21
• NCBI Protein Information: C-C motif chemokine 21; beta chemokine exodus-2; secondary lymphoid tissue chemokine; Efficient Chemoattractant for Lymphocytes; small inducible cytokine subfamily A (Cys-Cys), member 21
• UniProt Protein Name: C-C motif chemokine 21
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL21
• UniProt Synonym Gene Names: SCYA21; SLC
• UniProt Entry Name: CCL21_HUMAN