Rabbit CBX8 Polyclonal Antibody | anti-CBX8 antibody

CBX8 Polyclonal Antibody

Cat. Num. AAA3011406

• Gene Names: CBX8; PC3; RC1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CBX8; Polyclonal Antibody; CBX8 Polyclonal Antibody; Cbx8; CBX8_HUMAN; Chromobox Homolog 8 (Drosophila Pc Class); Chromobox Homolog 8 (Pc Class Homolog; Drosophila); Chromobox Homolog 8; Chromobox protein homolog 8; hPc3; Pc Class 3 Homolog (Drosophila); Pc Class 3 Homolog; Pc3; Polycomb 3; Polycomb 3 homolog; RC1; Rectachrome 1; anti-CBX8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CBX8 polyclonal antibody detects endogenous levels of CBX8 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 389

• Applicable Applications for anti-CBX8 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:10-1:100
• Immunogen: Recombinant full length Human CBX8.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CBX8 polyclonal antibod)

Related Product Information for anti-CBX8 antibody

Polycomb group (PcG) proteins form multiprotein complexes and play a role in gene silencing and Hox gene regulation by altering chromatin structure during transcription. The PcG protein MPc3 is also known as CBX8, PC3, HPc3 or RC1. MPc2 and MPc3 are PcG proteins that show structural similarity to M33 and, like M33, bind the PcG protein RING1 through a conserved c-box motif located in the C-terminus of RING1. However, MPc3 has only been shown to bind RING1 in vivo with covalently modified forms of RING1. MPc3 also interacts with the carboxy-terminus of AF9, a transcriptional activator implicated in the development of acute lukemias. MPc3 acts as a long range transcriptional silencer when targeted to a reporter gene by a heterologous DNA-binding domain. The human MPc3 gene maps to chromosome 17q25.3 and encodes a 389 amino acid protein.

NCBI and Uniprot Product Information

• NCBI GI #: 78099843
• NCBI GeneID: 57332
• UniProt Accession #: Q9HC52
• Molecular Weight: ~ 50kDa
• NCBI Official Full Name: Chromobox protein homolog 8
• NCBI Official Synonym Full Names: chromobox 8
• NCBI Official Symbol: CBX8
• NCBI Official Synonym Symbols: PC3; RC1
• NCBI Protein Information: chromobox protein homolog 8
• UniProt Protein Name: Chromobox protein homolog 8
• Protein Family: Chromobox protein
• UniProt Gene Name: CBX8
• UniProt Synonym Gene Names: PC3; RC1; Pc3; hPc3
• UniProt Entry Name: CBX8_HUMAN

Uniprot Description

CBX8: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; heterochromatin; nuclear chromatin; PcG protein complex; nucleus

Molecular Function: protein binding; single-stranded RNA binding; ubiquitin-protein ligase activity; methylated histone residue binding

Biological Process: transcription, DNA-dependent; histone ubiquitination; negative regulation of transcription from RNA polymerase II promoter