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Immunohistochemistry (IHC) (KCNJ9/Kir3.3 IHC staining of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Rabbit Kir3.3 Polyclonal Antibody

Kir3.3 (GIRK3, Kcnj9, G-protein Activated Inwardly Rectifying Potassium Channel)

Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
Kir3.3; Polyclonal Antibody; Kir3.3 (GIRK3; Kcnj9; G-protein Activated Inwardly Rectifying Potassium Channel); Anti -Kir3.3 (GIRK3; anti-Kir3.3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human KCNJ9/Kir3.3. Species Crossreactivity: Mouse, rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-Kir3.3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:40,000
Western Blot 1:500-1:1000
Immunohistochemistry (Paraffin): 5ug/ml
Immunogen
Synthetic peptide corresponding to human KCNJ9/Kir3.3.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(KCNJ9/Kir3.3 IHC staining of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (KCNJ9/Kir3.3 IHC staining of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Western blot analysis of extracts from LOVO cells, using KCNJ9. The lane on the right is treated with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of extracts from LOVO cells, using KCNJ9. The lane on the right is treated with the synthesized peptide.)
Related Product Information for anti-Kir3.3 antibody
This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium (By similarity).
Product Categories/Family for anti-Kir3.3 antibody

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Product Notes

The Kir3.3 (Catalog #AAA6000850) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Kir3.3 (GIRK3, Kcnj9, G-protein Activated Inwardly Rectifying Potassium Channel) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kir3.3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: ELISA: 1:40,000 Western Blot 1:500-1:1000 Immunohistochemistry (Paraffin): 5ug/ml. Researchers should empirically determine the suitability of the Kir3.3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kir3.3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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