Rabbit anti-Mouse Cbx5 Polyclonal Antibody | anti-Cbx5 antibody

Cbx5, CT (Cbx5, Hp1a, Chromobox protein homolog 5, Heterochromatin protein 1 homolog alpha) (Azide free) (HRP)

Cat. Num. AAA6281226

• Gene Names: Cbx5; HP1; Hp1a; C75991; Hp1alpha; 2610029O15Rik
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Cbx5; Polyclonal Antibody; CT (Cbx5; Hp1a; Chromobox protein homolog 5; Heterochromatin protein 1 homolog alpha) (Azide free) (HRP); Heterochromatin protein 1 homolog alpha; anti-Cbx5 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Cbx5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Cbx5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the C-terminal region of mouse Cbx5.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Cbx5 Antibody (C-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the Cbx5 antibody detected the Cbx5 protein (arrow).)

Related Product Information for anti-Cbx5 antibody

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins (By similarity).

Product Categories/Family for anti-Cbx5 antibody

Antibodies; Chromatin Proteins

References

Sun, Y., et al. Biophys. J. 99(4):1274-1283(2010). Muller, K.P., et al. Biophys. J. 97(11):2876-2885(2009). Dong, K.B., et al. EMBO J. 27(20):2691-2701(2008). Villeneuve, L.M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(26):9047-9052(2008). Brancorsini, S., et al. Cell Cycle 7(10):1415-1422(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 116008461
• NCBI GeneID: 12419
• NCBI Accession #: NP_001070257.1
• NCBI GenBank Nucleotide #: NM_001076789.1
• UniProt Accession #: Q61686; Q9CS35; Q9CXD1
• Molecular Weight: 22,186 Da
• NCBI Official Full Name: chromobox protein homolog 5
• NCBI Official Synonym Full Names: chromobox 5
• NCBI Official Symbol: Cbx5
• NCBI Official Synonym Symbols: HP1; Hp1a; C75991; Hp1alpha; 2610029O15Rik
• NCBI Protein Information: chromobox protein homolog 5; HP1 alpha; mHP1(alpha); chromobox homolog 5; heterochromatin protein 1 alpha; heterochromatin protein 1 homolog alpha
• UniProt Protein Name: Chromobox protein homolog 5
• Protein Family: Chromobox protein
• UniProt Gene Name: Cbx5
• UniProt Synonym Gene Names: Hp1a; HP1 alpha
• UniProt Entry Name: CBX5_MOUSE