Western Blot (WB)
(KRT28 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the KRT28 antibody detected the KRT28 protein (arrow).)
Rabbit anti-Human KRT28 Polyclonal Antibody | anti-KRT28 antibody
KRT28, CT (KRT28, KRT25D, Keratin, type I cytoskeletal 28, Cytokeratin-28, Keratin-25D, Keratin-28, Type I inner root sheath-specific keratin-K25irs4) (FITC)
Gene Names
KRT28; KRT25D
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
KRT28; Polyclonal Antibody; CT (KRT28; KRT25D; Keratin; type I cytoskeletal 28; Cytokeratin-28; Keratin-25D; Keratin-28; Type I inner root sheath-specific keratin-K25irs4) (FITC); Type I inner root sheath-specific keratin-K25irs4; anti-KRT28 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-KRT28 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
KRT28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 431-460 amino acids from the C-terminal region of human KRT28.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(KRT28 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the KRT28 antibody detected the KRT28 protein (arrow).)
Western Blot (WB)
(KRT28 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the KRT28 antibody detected the KRT28 protein (arrow).)
Related Product Information for anti-KRT28 antibody
This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21.
Product Categories/Family for anti-KRT28 antibody
References
Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006). Rogers, M.A., et al. Differentiation 72 (9-10), 527-540 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,567 Da
NCBI Official Full Name
keratin, type I cytoskeletal 28
NCBI Official Synonym Full Names
keratin 28
NCBI Official Symbol
KRT28
NCBI Official Synonym Symbols
KRT25D
NCBI Protein Information
keratin, type I cytoskeletal 28; K28; K25D; CK-28; keratin-28; keratin 25D; keratin-25D; cytokeratin-28; type I inner root sheath specific keratin 25 irs4; type I inner root sheath-specific keratin-K25irs4
UniProt Protein Name
Keratin, type I cytoskeletal 28
Protein Family
UniProt Gene Name
KRT28
UniProt Synonym Gene Names
KRT25D; CK-28; K25D; K28
UniProt Entry Name
K1C28_HUMAN
NCBI Description
This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2009]
Uniprot Description
KRT28: Essential for the proper assembly of types I and II keratin protein complexes and the formation of keratin intermediate filaments in the inner root sheath (irs). Belongs to the intermediate filament family.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21.2
Cellular Component: cytoplasm; intermediate filament
Molecular Function: structural molecule activity
Similar Products
Product Notes
The KRT28 krt28 (Catalog #AAA6314637) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRT28, CT (KRT28, KRT25D, Keratin, type I cytoskeletal 28, Cytokeratin-28, Keratin-25D, Keratin-28, Type I inner root sheath-specific keratin-K25irs4) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KRT28 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KRT28 krt28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
