Rabbit anti-Human CLC Polyclonal Antibody | anti-CLC antibody

CLC, CT (CLC, Eosinophil lysophospholipase, Charcot-Leyden crystal protein, Galectin-10, Lysolecithin acylhydrolase) (AP)

Cat. Num. AAA6286305

• Gene Names: CLC; GAL10; Gal-10; LGALS10; LGALS10A; LPPL_HUMAN
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CLC; Polyclonal Antibody; CT (CLC; Eosinophil lysophospholipase; Charcot-Leyden crystal protein; Galectin-10; Lysolecithin acylhydrolase) (AP); Lysolecithin acylhydrolase; anti-CLC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CLC antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-124 amino acids from the C-terminal region of human CLC.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CLC (arrow) using rabbit polyclonal CLC Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLC gene.)

Related Product Information for anti-CLC antibody

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine to glycerophosphocholine and a free fatty acid. This protein may possess carbohydrate or IgE-binding activities. It is both structurally and functionally related to the galectin family of beta-galactoside binding proteins. It may be associated with inflammation and some myeloid leukemias.

Product Categories/Family for anti-CLC antibody

Antibodies; Crystallins

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Bryborn, M., et al. Allergy 65(2):220-228(2010). De Re, V., et al. Ann. N. Y. Acad. Sci. 1173, 357-364 (2009): Than, N.G., et al. Proc. Natl. Acad. Sci. U.S.A. 106(24):9731-9736(2009). Kubach, J., et al. Blood 110(5):1550-1558(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 20357559
• NCBI GeneID: 1178
• NCBI Accession #: NP_001819.2
• NCBI GenBank Nucleotide #: NM_001828.5
• UniProt Accession #: Q05315; Q0VDE3; C5HZ13; C5HZ14
• Molecular Weight: 16,453 Da
• NCBI Official Full Name: galectin-10
• NCBI Official Synonym Full Names: Charcot-Leyden crystal galectin
• NCBI Official Symbol: CLC
• NCBI Official Synonym Symbols: GAL10; Gal-10; LGALS10; LGALS10A; LPPL_HUMAN
• NCBI Protein Information: galectin-10; Charcot-Leyden crystal protein; eosinophil lysophospholipase; galectin 10; lectin, galactoside-binding, soluble, 10; lysolecithin acylhydrolase
• UniProt Protein Name: Galectin-10
• Protein Family: Clathrin
• UniProt Gene Name: CLC
• UniProt Synonym Gene Names: LGALS10; LGALS10A; Gal-10; CLC
• UniProt Entry Name: LEG10_HUMAN

NCBI Description

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine to glycerophosphocholine and a free fatty acid. This protein may possess carbohydrate or IgE-binding activities. It is both structurally and functionally related to the galectin family of beta-galactoside binding proteins. It may be associated with inflammation and some myeloid leukemias. [provided by RefSeq, Jul 2008]

Uniprot Description

CLC: May have both lysophospholipase and carbohydrate-binding activities.

Protein type: Phospholipase; EC 3.1.1.5

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytosol

Molecular Function: protein binding; carbohydrate binding; lysophospholipase activity

Biological Process: regulation of activated T cell proliferation; regulation of T cell cytokine production; multicellular organismal development; regulation of T cell anergy