Rabbit anti-Human CATSPER2 Polyclonal Antibody | anti-CATSPER2 antibody

CATSPER2, CT (CATSPER2, Cation channel sperm-associated protein 2) (APC)

Cat. Num. AAA6281025

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CATSPER2; Polyclonal Antibody; CT (CATSPER2; Cation channel sperm-associated protein 2) (APC); Cation channel sperm-associated protein 2; anti-CATSPER2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CATSPER2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CATSPER2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 472-500 amino acids from the C-terminal region of human CATSPER2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CATSPER2 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the CATSPER2 antibody detected the CATSPER2 protein (arrow).)

Related Product Information for anti-CATSPER2 antibody

Calcium ions play a primary role in the regulation of sperm motility. This gene belongs to a family of putative cation channels that are specific to spermatozoa and localize to the flagellum. The protein family features a single repeat with six membrane-spanning segments and a predicted calcium-selective pore region. This gene is part of a tandem repeat on chromosome 15q15; the second copy of this gene is thought to be a pseudogene. Additional splice variants have been described but their full-length nature has not been determined.

Product Categories/Family for anti-CATSPER2 antibody

Antibodies; Ion Channel

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Lamesch, P., et al. Genomics 89(3):307-315(2007). Zhang, D., et al. J. Biol. Chem. 281(31):22332-22341(2006). Clapham, D.E., et al. Pharmacol. Rev. 57(4):451-454(2005). Quill, T.A., et al. Proc. Natl. Acad. Sci. U.S.A. 98(22):12527-12531(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 26051227
• NCBI GeneID: 117155
• UniProt Accession #: Q96P56
• NCBI Official Synonym Full Names: cation channel sperm associated 2
• NCBI Official Symbol: CATSPER2
• NCBI Protein Information: cation channel sperm-associated protein 2
• UniProt Protein Name: Cation channel sperm-associated protein 2
• Protein Family: Cation channel sperm-associated protein
• UniProt Gene Name: CATSPER2
• UniProt Synonym Gene Names: CatSper2
• UniProt Entry Name: CTSR2_HUMAN

NCBI Description

This gene encodes a member of a family of cation channel proteins that localize to the flagellum of spermatozoa. Defects at this locus causes male infertility. Alternatively spliced transcript variants have been observed at this locus. Readthrough transcription originates upstream of this locus in diphosphoinositol pentakisphosphate kinase 1 pseudogene 1 and is represented by GeneID:110006325. Related pseudogenes are found next to this locus on chromosome 15 and on chromosome 5. [provided by RefSeq, Mar 2017]

Uniprot Description

Function: Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. Activated by extracellular progesterone and prostaglandins following the sequence: progesterone > PGF1-alpha = PGE1 > PGA1 > PGE2 >> PGD2. The primary effect of progesterone activation is to shift voltage dependence towards more physiological, negative membrane potentials; it is not mediated by metabotropic receptors and second messengers. Sperm capacitation enhances the effect of progesterone by providing additional negative shift. Also activated by the elevation of intracellular pH. Ref.7 Ref.8

Subunit structure: Heterotetramer; possibly composed of CATSPER1, CATSPER2, CATSPER3 and CATSPER4

Potential. Component of the CatSper complex. Interacts with Ca(v)3.3/CACNA1I, leading to suppress T-type calcium channel activity. Ref.4

Subcellular location: Cell projection › cilium › flagellum membrane; Multi-pass membrane protein

By similarity. Note: Specifically located in the principal piece of sperm tail.

Tissue specificity: Testis-specific. Ref.1 Ref.6

Involvement in disease: Deafness-infertility syndrome (DIS) [MIM:611102]: Characterized by deafness and infertility and is caused by large contiguous gene deletions at 15q15.3 that removes both STRC and CATSPER2 genes.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.3 Ref.5

Sequence similarities: Belongs to the cation channel sperm-associated (TC 1.A.1.19) family. [View classification]