Rabbit anti-Human CATSPER1 Polyclonal Antibody | anti-CATSPER1 antibody

CATSPER1, NT (CATSPER1, Cation channel sperm-associated protein 1) (FITC)

Cat. Num. AAA6281016

• Gene Names: CATSPER1; SPGF7; CATSPER
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CATSPER1; Polyclonal Antibody; NT (CATSPER1; Cation channel sperm-associated protein 1) (FITC); Cation channel sperm-associated protein 1; anti-CATSPER1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CATSPER1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CATSPER1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-65 amino acids from the N-terminal region of human CATSPER1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CATSPER1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CATSPER1 antibody detected the CATSPER1 protein (arrow).)

Related Product Information for anti-CATSPER1 antibody

Calcium ions play a primary role in the regulation of sperm motility. This gene belongs to a family of putative cation channels that are specific to spermatozoa and localize to the flagellum. The protein family features a single repeat with six membrane-spanning segments and a predicted calcium-selective pore region.

Product Categories/Family for anti-CATSPER1 antibody

Antibodies; Ion Channel

References

Li, H., et al. Fertil. Steril. 92(3):1141-1146(2009). Avenarius, M.R., et al. Am. J. Hum. Genet. 84(4):505-510(2009). Zhang, D., et al. J. Biol. Chem. 281(31):22332-22341(2006). Li, H.G., et al. Asian J. Androl. 8(3):301-306(2006). Clapham, D.E., et al. Pharmacol. Rev. 57(4):451-454(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 221316609
• NCBI GeneID: 117144
• NCBI Accession #: NP_444282.3
• NCBI GenBank Nucleotide #: NM_053054.3
• UniProt Accession #: Q8NEC5; Q96P76
• Molecular Weight: 90,091 Da
• NCBI Official Full Name: cation channel sperm-associated protein 1
• NCBI Official Synonym Full Names: cation channel, sperm associated 1
• NCBI Official Symbol: CATSPER1
• NCBI Official Synonym Symbols: SPGF7; CATSPER
• NCBI Protein Information: cation channel sperm-associated protein 1
• UniProt Protein Name: Cation channel sperm-associated protein 1
• Protein Family: Cation channel sperm-associated protein
• UniProt Gene Name: CATSPER1
• UniProt Synonym Gene Names: CatSper1; hCatSper
• UniProt Entry Name: CTSR1_HUMAN

NCBI Description

Calcium ions play a primary role in the regulation of sperm motility. This gene belongs to a family of putative cation channels that are specific to spermatozoa and localize to the flagellum. The protein family features a single repeat with six membrane-spanning segments and a predicted calcium-selective pore region. [provided by RefSeq, Jul 2008]

Uniprot Description

CATSPER1: Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. Activated by extracellular progesterone and prostaglandins following the sequence: progesterone > PGF1-alpha = PGE1 > PGA1 > PGE2 >> PGD2. The primary effect of progesterone activation is to shift voltage dependence towards more physiological, negative membrane potentials; it is not mediated by metabotropic receptors and second messengers. Sperm capacitation enhances the effect of progesterone by providing additional negative shift. Also activated by the elevation of intracellular pH. Defects in CATSPER1 are the cause of spermatogenic failure type 7 (SPGF7). An infertility disorder characterized by non-motile sperm or sperm motility below the normal threshold, low sperm count, increased abnormally structured spermatozoa, and reduced semen volume. Belongs to the cation channel sperm-associated (TC 1.A.1.19) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: plasma membrane

Molecular Function: voltage-gated calcium channel activity; protein binding; calcium activated cation channel activity

Biological Process: sperm-egg recognition; sperm motility; fusion of sperm to egg plasma membrane; multicellular organismal development; single fertilization; spermatogenesis; regulation of calcium ion transport; cell differentiation; multicellular organism reproduction

Disease: Spermatogenic Failure 7