Immunohistochemistry (IHC)
(Anti-Cathepsin D Picoband antibody, MBS176922-1.JPGIHC(P): Mouse Kidney Tissue )
Rabbit Cathepsin D Polyclonal Antibody | anti-CTSD antibody
Anti-Cathepsin D Antibody
Gene Names
Ctsd; CD; CatD
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
Cathepsin D; Polyclonal Antibody; Anti-Cathepsin D Antibody; cathepsin D; CatD antibody; CATD_HUMAN antibody; Cathepsin D heavy chain antibody; Cathepsin D light chain antibody; CathepsinD antibody; CD antibody; Ceroid lipofuscinosis neuronal 10 antibody; CLN10 antibody; CPSD antibody; CTSD antibody; Lysosomal aspartyl peptidase antibody; Lysosomal aspartyl protease antibody; MGC2311 antibody; anti-CTSD antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
410
Applicable Applications for anti-CTSD antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
WB: Concentration: 0.1-0.5ug/ml; Tested Species: Mouse
IHC-P: Concentration: 0.5-1ug/ml; Tested Species: Mouse, Rat; Antigen Retieval: By Heat
WB: The detection limit for cathepsin D is approximately 0.25ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin section 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit MBS176460 in WB, supported by MBS176451 in IHC-P.
IHC-P: Concentration: 0.5-1ug/ml; Tested Species: Mouse, Rat; Antigen Retieval: By Heat
WB: The detection limit for cathepsin D is approximately 0.25ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin section 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit MBS176460 in WB, supported by MBS176451 in IHC-P.
Immunogen
E Coli-derived mouse Cathepsin D recombinant protein (Position: E65-L410). Mouse Cathepsin D shares 85% and 91% amino acid (aa) sequences identity with human and rat Cathepsin D, respectively.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Species
Mouse, Rat
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Immunohistochemistry (IHC)
(Anti-Cathepsin D Picoband antibody, MBS176922-1.JPGIHC(P): Mouse Kidney Tissue )
Immunohistochemistry (IHC)
(Anti-Cathepsin D Picoband antibody, MBS176922-1.JPGIHC(P): Mouse Kidney Tissue )
Immunohistochemistry (IHC)
(Anti-Cathepsin D Picoband antibody, MBS176922-2.JPGIHC(P): Mouse Liver Tissue )
Immunohistochemistry (IHC)
(Anti-Cathepsin D Picoband antibody, MBS176922-2.JPGIHC(P): Mouse Liver Tissue )
Testing Data
(Anti-Cathepsin D Picoband antibody, MBS176922-3.jpgAll lanes: Anti-Cathepsin D(MBS176922) at 0.5ug/mlLane 1: Mouse Liver Tissue Lysate at 40ugLane 2: Mouse Brain Tissue Lysate at 40ugLane 3: Mouse Thymus Tissue Lysate at 40ugLane 4: NEURO Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD )
Testing Data
(Anti-Cathepsin D Picoband antibody, MBS176922-3.jpgAll lanes: Anti-Cathepsin D(MBS176922) at 0.5ug/mlLane 1: Mouse Liver Tissue Lysate at 40ugLane 2: Mouse Brain Tissue Lysate at 40ugLane 3: Mouse Thymus Tissue Lysate at 40ugLane 4: NEURO Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD )
Related Product Information for anti-CTSD antibody
Description: Rabbit IgG polyclonal antibody for Cathepsin D(CTSD) detection. Tested with WB, IHC-P in Mouse, Rat.
Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Cathepsin D
NCBI Official Synonym Full Names
cathepsin D
NCBI Official Symbol
Ctsd
NCBI Official Synonym Symbols
CD; CatD
NCBI Protein Information
cathepsin D
UniProt Protein Name
Cathepsin D
Protein Family
UniProt Gene Name
Ctsd
UniProt Entry Name
CATD_MOUSE
Uniprot Description
CTSD: Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. Consists of a light chain and a heavy chain. Belongs to the peptidase A1 family.
Protein type: Autophagy; Motility/polarity/chemotaxis; Protease; EC 3.4.23.5
Cellular Component: extracellular matrix; extracellular space; mitochondrion; lysosome; extracellular region
Molecular Function: peptidase activity; hydrolase activity; endopeptidase activity; aspartic-type endopeptidase activity; peptide binding
Biological Process: proteolysis; autophagic vacuole formation
Research Articles on CTSD
Similar Products
Product Notes
The CTSD ctsd (Catalog #AAA176922) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cathepsin D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: Concentration: 0.1-0.5ug/ml; Tested Species: Mouse IHC-P: Concentration: 0.5-1ug/ml; Tested Species: Mouse, Rat; Antigen Retieval: By Heat WB: The detection limit for cathepsin D is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin section 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Antibody can be supported by chemiluminescence kit MBS176460 in WB, supported by MBS176451 in IHC-P. . Researchers should empirically determine the suitability of the CTSD ctsd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cathepsin D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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