Rabbit Caspase-9 Polyclonal Antibody | anti-CASP9 antibody

Caspase-9 Antibody

Cat. Num. AAA415740

• Gene Names: CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• Reactivity: Human, mouse, rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Caspase-9; Polyclonal Antibody; Caspase-9 Antibody; anti-CASP9 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This Polyclonal antibody is directed against human Caspase-9 protein. The product was purified from seurm by protein A chromatography.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 266

• Applicable Applications for anti-CASP9 antibody: Western Blot (WB)
• Immunogen: The anti-caspase-9 polyclonal antibody was raised against a peptide corresponding to amino acids 299 to 318 of human caspase-9.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Related Product Information for anti-CASP9 antibody

Caspase-9 is a Rabbit polyclonal to Caspase-9

References

1. Duan, H., Orth, K., Chinnaiyan, A.M., et al.ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J. Biol. Chem.271, 16720-16724 (1996).2. Li, P., Nijhawan, D., Budihardjo, I., et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 91, 479-489 (1997).3. Srinivasula, S.M., Fernandes-Alnemri, T., Zangrilli, J., et al. The Ced-3/interleukin 1 beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2beta are substrates for the apoptotic mediator CPP32. J. Biol. Chem.271, 27099-27106 (1996).4. Pan, G., O’Rourke, K., and Dixit, V.M. Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex. J. Biol. Chem.273, 5841-5845 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 6518502
• NCBI GeneID: 842
• UniProt Accession #: P55211; O95348; Q53Y70; Q5JRU9; Q5UGI1; Q92852; Q9BQ62; Q9UEQ3; Q9UIJ8; B4E1A3
• Molecular Weight: 46,281 Da
• NCBI Official Full Name: caspase-9
• NCBI Official Synonym Full Names: caspase 9, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP9
• NCBI Official Synonym Symbols: MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• NCBI Protein Information: caspase-9; apoptotic protease MCH-6; ICE-like apoptotic protease 6; apoptotic protease activating factor 3; protein phosphatase 1, regulatory subunit 56
• UniProt Protein Name: Caspase-9
• Protein Family: Caspase
• UniProt Gene Name: CASP9
• UniProt Synonym Gene Names: MCH6; CASP-9; APAF-3; ICE-LAP6
• UniProt Entry Name: CASP9_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Ref.17 Ref.24Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. Ref.17 Ref.24

Catalytic activity: Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-|-Xaa. Ref.24

Enzyme regulation: Inhibited by the effector protein NleF that is produced by pathogenic E.coli; this inhibits apoptosis. Ref.24

Subunit structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts (inactive form) with EFHD2. Interacts with HAX1. Interacts with BIRC2/c-IAP1, XIAP/BIRC4, BIRC5/survivin, BIRC6/bruce and BIRC7/livin. Interacts with ABL1 (via SH3 domain); the interaction is direct and increases in the response of cells to genotoxic stress and ABL1/c-Abl activation. Interacts with NleF from pathogenic E.coli. Ref.16 Ref.17 Ref.18 Ref.20 Ref.22 Ref.24

Tissue specificity: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. Ref.18

Developmental stage: Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart. Ref.18

Post-translational modification: Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage. Ref.15 Ref.17

Sequence similarities: Belongs to the peptidase C14A family.Contains 1 CARD domain.