Rabbit anti-Human Caspase-6 Polyclonal Antibody | anti-CASP6 antibody

Caspase-6 p18 Polyclonal Antibody

Cat. Num. AAA3012363

• Gene Names: CASP6; MCH2
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: Caspase-6; Polyclonal Antibody; Caspase-6 p18 Polyclonal Antibody; CASP-6; CASP6; Apoptotic protease Mch-2; Apoptotic protease MCH2; Caspase 6; Caspase 6 apoptosis related cysteine protease; apoptosis related cysteine peptidase; Caspase-6 subunit p11; Caspase-6 subunit p20; Mch2; OTTHUMP00000162742; OTTHUMP00000162743; anti-CASP6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Caspase-6 p18 polyclonal antibody detects endogenous levels of Caspase-6 p18 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 293

• Applicable Applications for anti-CASP6 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:1,000-1:5,000
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Caspase-6 p18 on U937 cells lysates using anti-Caspase-6 p18 antibody at 1/1,000 dilution.)

Related Product Information for anti-CASP6 antibody

A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.

NCBI and Uniprot Product Information

• NCBI GI #: 14916483
• NCBI GeneID: 839
• NCBI Accession #: NP_001217.2
• NCBI GenBank Nucleotide #: NP_001217.2
• UniProt Accession #: P55212
• Molecular Weight: 18/33kDa
• NCBI Official Full Name: caspase-6 isoform alpha
• NCBI Official Synonym Full Names: caspase 6
• NCBI Official Symbol: CASP6
• NCBI Official Synonym Symbols: MCH2
• NCBI Protein Information: caspase-6
• UniProt Protein Name: Caspase-6
• Protein Family: Caspase
• UniProt Gene Name: CASP6
• UniProt Synonym Gene Names: MCH2; CASP-6
• UniProt Entry Name: CASP6_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Oct 2015]

Uniprot Description

CASP6: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Activation is suppressed by phosphorylation at Ser-257. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.59; Protease; Apoptosis

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: epithelial cell differentiation; apoptosis; proteolysis; cell structure disassembly during apoptosis