Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using caspase-4 antibody at 1:200 dilution.)
Rabbit Caspase 4 Polyclonal Antibody | anti-CASP-4 antibody
Caspase 4 Antibody
Gene Names
Casp4; Casp11
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
Caspase 4; Polyclonal Antibody; Caspase 4 Antibody; Affinity Purified Rabbit Polyclonal Caspase 4 Antibody; Polyclonal Caspase 4 antibody; Anti-Caspase 4 antibody; Caspase -4 antibody; caspase 4 apoptosis-related cysteine peptidase antibody; protease TX antibody; caspase-4 antibody; Caspase 4 antibody; ICEREL-II antibody; CASP-4 antibody; Caspase -4; ICE(rel)II antibody; TX antibody; apoptotic cysteine protease Mih1/TX antibody; ICE(rel)-II antibody; CASP4 antibody; caspase 4 apoptosis-related cysteine protease antibody; ICH-2 antibody; protease ICH-2 antibody; Mih1/TX antibody; anti-CASP-4 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
373
Applicable Applications for anti-CASP-4 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:200
ICC: 1:100-1:200
IHC-P: 1:100-1:500
WB: 1:500-1:3000
ICC: 1:100-1:200
IHC-P: 1:100-1:500
WB: 1:500-1:3000
Immunogen
Caspase 4 antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 68 and 162 of Caspase 4
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using caspase-4 antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using caspase-4 antibody at 1:200 dilution.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 10% SDS PAGE gel and Caspase 4 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 10% SDS PAGE gel and Caspase 4 antibody at a dilution of 1:1000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded SAS xenograft using Caspase 4 antibody at a dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded SAS xenograft using Caspase 4 antibody at a dilution of 1:500)
Related Product Information for anti-CASP-4 antibody
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis.
Product Categories/Family for anti-CASP-4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43 kDa
NCBI Official Full Name
caspase 4
NCBI Official Synonym Full Names
caspase 4
NCBI Official Symbol
Casp4
NCBI Official Synonym Symbols
Casp11
NCBI Protein Information
caspase 4
NCBI Description
cysteine-aspartic acid protease (caspase); involved with the terminal stage of apoptosis; may also be involved with response to inflammation [RGD, Feb 2006]
Research Articles on CASP-4
Similar Products
Product Notes
The CASP-4 (Catalog #AAA5311751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Caspase 4 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:200 ICC: 1:100-1:200 IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the CASP-4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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