Rabbit Caspase 2 Polyclonal Antibody | anti-K152 antibody

Caspase 2 (K152) Polyclonal Antibody

Cat. Num. AAA3003287

• Gene Names: CASP2; ICH1; NEDD2; CASP-2; NEDD-2; PPP1R57
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Caspase 2; Polyclonal Antibody; Caspase 2 (K152) Polyclonal Antibody; Caspase-2; Caspase2; CASP-2; Neural precursor cell expressed developmentally down-regulated protein 2; NEDD-2; Protease ICH-1; CASP2; ICH1; NEDD2; anti-K152 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Caspase 2 (K136) polyclonal antibody detects endogenous levels of Caspase 2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 312

• Applicable Applications for anti-K152 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 140-180 of Human Caspase 2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Caspase 2 (K152) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-K152 antibody

Caspase-2 (Nedd2, ICH-1) is an aspartate-specific cysteine protease that is activated in response to various apoptotic stimuli. Caspase-2 is unique among the caspases in that it has features of both upstream caspases (long prodomain) and downstream caspases (DEXD substrate specificity). Caspase-2 is highly expressed in the brain during development and is expressed at low levels in adult tissue. Specifically, caspase-2 localizes to the mitochondria, the Golgi, the cytoplasm and the nucleus. Caspase-2 exists as two isoforms, caspase-2L and caspase-2S, which are produced by alternative splicing and differ in their N- and C-termini. Caspase-2L acts as a positive regulator of apoptosis, whereas caspase-2S functions as a negative regulator of apoptosis. Following apoptotic stimuli, the caspase-2L precursor undergoes cleavage at Asp 153 to produce a fragment (p30). The p30 fragment undergoes further cleavage to generate a fragment containing amino acids 153-308 (p18) and a fragment containing amino acids 317-435 (p13 or p14). As apoptosis progresses, the p13 (p14) fragment can undergo further processing to yield a fragment containing amino acids 331-435 (p12).

NCBI and Uniprot Product Information

• NCBI GI #: 4502575
• NCBI GeneID: 835
• NCBI Accession #: NP_001215.1
• NCBI GenBank Nucleotide #: NP_001215.1
• UniProt Accession #: P42575
• Molecular Weight: ~ 51kDa
• NCBI Official Full Name: caspase-2 isoform 2
• NCBI Official Synonym Full Names: caspase 2
• NCBI Official Symbol: CASP2
• NCBI Official Synonym Symbols: ICH1; NEDD2; CASP-2; NEDD-2; PPP1R57
• NCBI Protein Information: caspase-2
• UniProt Protein Name: Caspase-2
• UniProt Gene Name: CASP2
• UniProt Synonym Gene Names: ICH1; NEDD2; CASP-2; NEDD-2
• UniProt Entry Name: CASP2_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Caspases mediate cellular apoptosis through the proteolytic cleavage of specific protein substrates. The encoded protein may function in stress-induced cell death pathways, cell cycle maintenance, and the suppression of tumorigenesis. Increased expression of this gene may play a role in neurodegenerative disorders including Alzheimer's disease, Huntington's disease and temporal lobe epilepsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

CASP2: Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD. Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.4.22.55; Protease

Chromosomal Location of Human Ortholog: 7q34-q35

Cellular Component: membrane; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; cysteine-type endopeptidase activity

Biological Process: nerve growth factor receptor signaling pathway; luteolysis; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; positive regulation of apoptosis; positive regulation of neuron apoptosis; germ cell programmed cell death; protein processing; brain development; regulation of caspase activity; proteolysis; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; aging