Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse NADPH oxidase 3 (NOX3) Standard Curve.)
Mouse NADPH Oxidase 3 (NOX3) ELISA Kit | NOX3 elisa kit
Mouse NADPH Oxidase 3 (NOX3) ELISA Kit
Gene Names
Nox3; het; GP91-3; nmf250
Reactivity
Mouse
Synonyms
NADPH Oxidase 3 (NOX3); Mouse NADPH Oxidase 3 (NOX3) ELISA Kit; NOX3; GP91-3; NADPH oxidase catalytic subunit-like 3; NOX3 elisa kit
Reactivity
Mouse
Specificity
Mouse NADPH oxidase 3 (NOX3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NOX3. No significant cross-reactivity or interference between Mouse NOX3 and analogues was observed.
Sequence Length
568
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse NADPH oxidase 3 (NOX3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse NADPH oxidase 3 (NOX3) Standard Curve.)
Related Product Information for NOX3 elisa kit
Description: This Mouse NADPH oxidase 3 (NOX3) ELISA Kit employs a two-site sandwich ELISA to quantitate NOX3 in samples. An antibody specific for NOX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NOX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOX3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NOX3 is a member of the NOX family of NADPH oxidases. These enzymes have the capacity to generate superoxide and other reactive oxygen species (ROS) and transport electrons across the plasma membrane. The ROS generated by family members have been implicated in numerous biological functions including host defense, posttranlational processing of proteins, cellular signaling, regulation of gene expression, and cell differentiation. The protein encoded by this gene is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly.
Background: NOX3 is a member of the NOX family of NADPH oxidases. These enzymes have the capacity to generate superoxide and other reactive oxygen species (ROS) and transport electrons across the plasma membrane. The ROS generated by family members have been implicated in numerous biological functions including host defense, posttranlational processing of proteins, cellular signaling, regulation of gene expression, and cell differentiation. The protein encoded by this gene is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
NADPH oxidase 3
NCBI Official Synonym Full Names
NADPH oxidase 3
NCBI Official Symbol
Nox3
NCBI Official Synonym Symbols
het; GP91-3; nmf250
NCBI Protein Information
NADPH oxidase 3
UniProt Protein Name
NADPH oxidase 3
Protein Family
UniProt Gene Name
Nox3
UniProt Entry Name
NOX3_MOUSE
NCBI Description
This gene encodes a member of the NOX family of NADPH oxidases. These enzymes catalyze the transfer of electrons from NADPH to molecular oxygen to produce superoxide and other reactive oxygen species (ROS). The ROS generated by family members have been implicated in numerous biological functions including host defense, posttranlational processing of proteins, cellular signaling, regulation of gene expression, and cell differentiation. The protein encoded by this gene is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia, which are crystalline structures of the inner ear involved in the perception of gravity and linear acceleration. In mouse mutations of this gene lead to the absence of otoconia and vestibular dysfunction. [provided by RefSeq, Jun 2013]
Research Articles on NOX3
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Product Notes
The Mouse NOX3 nox3 (Catalog #AAA9718068) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718068 ELISA Kit recognizes Mouse NOX3. It is sometimes possible for the material contained within the vial of "NADPH Oxidase 3 (NOX3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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