Rabbit Caspase-10/FLICE2 Polyclonal Antibody | anti-CASP10 antibody

Polyclonal Antibody to Caspase-10/FLICE2

Cat. Num. AAA668567

• Gene Names: CASP10; MCH4; ALPS2; FLICE2
• Reactivity: Dog, Gerbil, Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Serum
• Synonyms: Caspase-10/FLICE2; Polyclonal Antibody; Polyclonal Antibody to Caspase-10/FLICE2; CASP10; MCH4; Apoptotic protease Mch-4; FLICE2; FAS-associated death domain protein interleukin-1B-converting enzyme 2; ICE-like apoptotic protease 4; Antibody to Caspase-10/FLICE2; Anti-Caspase-10/FLICE2 Antibody; Caspase-10/FLICE2 Antibody; anti-CASP10 antibody

• Host: Rabbit
• Reactivity: Dog, Gerbil, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Serum
• Form/Format: 50 ul sera
• Sequence Length: 521

• Applicable Applications for anti-CASP10 antibody: Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP)
• Application Notes: WB: 1: 1000-1: 2000; IHC (paraffin): 1: 1000-1: 5000; IP: 1: 50-1: 200
• Immunogen Information: A synthetic peptide of Caspase-10/FLICE2 protein (amino acids 435-455 QPAFTLRKKLVFPVPLDALSI) was used as the immunogen for this antibody
• Antigen: Caspase-10/FLICE2
• Preparation and Storage: Store the antibody at 4 degree C, stable for 6 months.; For long-term storage, store at -20 degree C.; Avoid repeated freeze and thaw cycles.

Related Product Information for anti-CASP10 antibody

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are constitutively expressed in almost all cell types as inactive proenzymes (zymogens: enzyme precursors which require a biochemical change to become active enzymes) that are processed and activated in response to a variety of pro-apoptotic or inflammatory stimuli. The procaspases (32-56 kDa) contain four domains: an N-terminal prodomain (2-25 kDa), a large subunit (p20: 17-21 kDa), a small subunit (p10: 10-13 kDa) and a short linker region between the large and small subunits. Caspase activation involves proteolytic processing of the proenzyme at specific aspartate residues between the domains. This results in removal of the prodomain as well as the linker region and formation of a heterodimer containing one large and one small subunit (p20-p10). The active caspase is a tetramer composed of two heterodimers (p202-p102). Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. Caspase-10 is a 455 amino acid protein. However, caspase-10 produces, by alternative splicing, at least 12 different transcripts, all with introns, putatively encoding at least 11 different protein isoforms. For example, human caspase-10a is a 479 amino acid (aa) protein, and human caspase-10d is a 522 aa protein. The transcripts appear to differ by truncation of the 3'end, presence or absence of 6 cassette exons, or common exons with different boundaries, because an internal intron is not always spliced out. Users are encoraged to go to the NCBI ACE View Data Base for more information. http: //www.ncbi.nih.gov/IEB/Research/Acembly/ Caspase-10 gene mutations or polymorphisms have been associated with a number of diseases including type II autoimmune lymphoproliferative syndrome, non-Hiodgkin's lymphoma, and gastric cancer. It is thought that some mutations of the caspase-10 gene may lead to the loss of its apoptotic function, thereby contributing to the pathogenesis of some diseases. Caspase-10 may be observed on Western blots at varying apparent molecular weights depending on isoform, truncation, or cleavage forms present. The antisera recognize caspase-10 forms that contain the peptide immunogen sequence (QPAFTLRKKLVFPVPLDALSI).

Product Categories/Family for anti-CASP10 antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 12644463
• NCBI GeneID: 843
• UniProt Accession #: Q92851; Q68HC0; Q6KF62; Q6KF63; Q8IUP5; Q8WYQ8; Q99845; Q9Y2U6; Q9Y2U7
• Molecular Weight: 28,364 Da
• NCBI Official Full Name: Caspase-10
• NCBI Official Synonym Full Names: caspase 10
• NCBI Official Symbol: CASP10
• NCBI Official Synonym Symbols: MCH4; ALPS2; FLICE2
• NCBI Protein Information: caspase-10
• UniProt Protein Name: Caspase-10
• Protein Family: Caspase
• UniProt Gene Name: CASP10
• UniProt Synonym Gene Names: MCH4; CASP-10; FLICE2

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

CASP10: Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit. Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon. Belongs to the peptidase C14A family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.63; Protease

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: CD95 death-inducing signaling complex; cytosol

Molecular Function: cysteine-type endopeptidase activity; protein binding; ubiquitin protein ligase binding

Biological Process: apoptosis; cell surface receptor linked signal transduction; induction of apoptosis via death domain receptors; macrophage differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of apoptosis

Disease: Autoimmune Lymphoproliferative Syndrome, Type Iia; Gastric Cancer; Lymphoma, Non-hodgkin, Familial