Mouse anti-Human RBBP6 Monoclonal Antibody | anti-RBBP6 antibody

Anti-RBBP6 Antibody (clone 5A11)

Cat. Num. AAA2401157

• Gene Names: RBBP6; PACT; MY038; P2P-R; RBQ-1; SNAMA
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified from ascites by Protein A
• Synonyms: RBBP6; Monoclonal Antibody; Anti-RBBP6 Antibody (clone 5A11); Rabbit Polyclonal (IgG) to Human RBBP6; p2P-R; PACT; RBQ1; MY038; p2PR; Protein P2P-R; RB-binding Q-protein 1; RBQ-1; SNAMA; anti-RBBP6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: 5A11
• Specificity: Human RBBP6
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4.
• Sequence Length: 1792

• Applicable Applications for anti-RBBP6 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF: 10 ug/ml; IHC-P: 1:50
• Immunogen: RBBP6 (NP_008841, 1582 a.a. ~ 1692 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C or lower.; Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to RBBP6 on HeLa cell. [antibody concentration 10 ug/ml].)

ELISA (EIA)

(Detection limit for recombinant GST tagged RBBP6 is approximately 0.1 ng/ml as a capture antibody.)

Related Product Information for anti-RBBP6 antibody

retinoblastoma tumor suppressor (pRB) protein binds with many otherproteins. In various human cancers, pRB suppresses cellularproliferation and is inactivated. Cell cycle-dependent phosphorylationregulates the activity of pRB. This gene encodes a protein which bindsto underphosphorylated but not phosphorylated pRB. Multiplealternatively spliced transcript variants that encode different isoformshave been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 33620769
• NCBI GeneID: 5930
• NCBI Accession #: NP_008841.2
• NCBI GenBank Nucleotide #: NM_006910.4
• UniProt Accession #: Q7Z6E9; Q147T5; Q15290; Q6DKH4; Q6P4C2; Q6YNC9; Q7Z6E8; Q8N0V2; Q96PH3; Q9H3I8; Q9H5M5; Q9NPX4
• Molecular Weight: 106,037 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RBBP6 isoform 1
• NCBI Official Synonym Full Names: RB binding protein 6, ubiquitin ligase
• NCBI Official Symbol: RBBP6
• NCBI Official Synonym Symbols: PACT; MY038; P2P-R; RBQ-1; SNAMA
• NCBI Protein Information: E3 ubiquitin-protein ligase RBBP6
• UniProt Protein Name: E3 ubiquitin-protein ligase RBBP6
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RBBP6
• UniProt Synonym Gene Names: P2PR; PACT; RBQ1; RBQ-1

NCBI Description

The retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. This gene encodes a protein which binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome (PubMed:18851979). May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth (). Regulates DNA-replication and the stability of chromosomal common fragile sites (CFSs) in a ZBTB38- and MCM10-dependent manner. Controls ZBTB38 protein stability and abundance via ubiquitination and proteasomal degradation, and ZBTB38 in turn negatively regulates the expression of MCM10 which plays an important role in DNA-replication (PubMed:24726359).