Rabbit anti-Human Caspase 1 Polyclonal Antibody | anti-CASP1 antibody

Caspase 1 (Interleukin-1Beta Converting Enzyme, ICE) (MaxLight 405)

Cat. Num. AAA6467454

• Gene Names: CASP1; ICE; P45; IL1BC
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Caspase 1; Polyclonal Antibody; Caspase 1 (Interleukin-1Beta Converting Enzyme; ICE) (MaxLight 405); anti-CASP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the p20 subunit of Caspase 1 and recognizes the P45 proenzyme (~45kD) and the p20 subunit (~20kD) of the reduced, human Caspase 1 isoform. May cross-react with Caspase 5 due to 92% sequence homology. Species sequence homology: mouse, rat, canine, porcine and feline (92% sequence homology).
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-CASP1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 5ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to the p20 subunit of human Caspase 1 conjugated with KLH.
• Conjugate: MaxLight405
• Positive Control: THP cell lysate
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(THP-1 cell lysate (10ug) was probed with C2086-68Q (5ug/mL). Proteins were visualized using a donkey anti-rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.)

Immunostaining (IS)

(Formalin Fixed Paraffin Embedded (FFPE) human spleen (tissues were processed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of C2086-68Q. Reactivity was detected using an anti-Rabbit secondary antibody and HRP-DAB. Positive cytoplasmic staining was observed in leukocytes of human spleen tissue.)

Immunostaining (IS)

(Formalin Fixed Paraffin Embedded (FFPE) human colon tissues were processed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:4,000 dilution of C2086-68Q. Reactivity was detected using an Anti-Rabbit secondary antibody and HRP-DAB. Strong basolateral staining, along with some diffuse cytoplasmic staining, was observed in crypt cells of human colon tissues.)

Immunostaining (IS)

(Formalin Fixed Paraffin Embedded (FFPE) human colon tissues were processed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:4,000 dilution of C2086-68Q. Reactivity was detected using an Anti-Rabbit secondary antibody and HRP-DAB. Strong basolateral staining, along with some diffuse cytoplasmic staining, was observed in crypt cells of human colon tissues.)

Product Categories/Family for anti-CASP1 antibody

Antibodies; Apoptosis; Caspase

References

1. Black, R.A., et al., FEBS Lett. 247: 386-390 (1989). 2. Kostura, M.J., et al., Proc. Natl. Acad. Sci. USA 86: 5227-5231 (1989). 3. Cerreti, D.P., et al., Science 256: 97-100 (1992). 4. Thornberry, N.A., et al., Nature 356: 768-774 (1992). 5. Wilson, K.P., et al., Nature 370: 270-275 (1994). 6. Walker, N.P.C., et al., Cell 78: 343-352 (1994). 7. Sealth, P.R., et al., J. Biol. Chem. 265: 14, 526-14, 528 (1990). 8. Howard, A.D., et al., J. Immunol. 147: 2964-2969 (1991). 9. Kumar, S., et al., Genes & Dev. 8: 1613-1626 (1994). 10. Yuan, J., et al., Cell 75: 641-652 (1993). 11. Miura, M., et al., Cell 75: 653-660 (1993). 12. Ray, C., et al., Cell 69: 597-604 (1992). 13. Gagliardini, V., et al., Science 263: 826-828 (1994). 14. Molineaux, et al., Proc. Natl. Acad. Sci. USA 90: 1809-1813 (1993). 15. Alnemvx, et al., J. Biol. Chem. 270: 4312-4317 (1995). 16. Martini, J., et al., Mol. Endocrinol. 14: 1536-1549 (2000). 17. Takeuchi, R., et al., J. Virol. 72: 4498-4502 (1998). 18. Cohen, G.M., Biochem. J. 326: 1-16 (1997). 19. Molineux, et al., PNAS 90: 1809 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 4502573
• NCBI GeneID: 834
• NCBI Accession #: NP_001214
• NCBI GenBank Nucleotide #: NM_001223
• UniProt Accession #: P29466
• Molecular Weight: 10kDa
• NCBI Official Full Name: caspase-1 isoform beta
• NCBI Official Synonym Full Names: caspase 1
• NCBI Official Symbol: CASP1
• NCBI Official Synonym Symbols: ICE; P45; IL1BC
• NCBI Protein Information: caspase-1
• UniProt Protein Name: Caspase-1
• UniProt Gene Name: CASP1
• UniProt Synonym Gene Names: IL1BC; IL1BCE; CASP-1; IL-1BC; ICE
• UniProt Entry Name: CASP1_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

Uniprot Description

CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.36; Protease; Apoptosis

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: cytoplasm; extracellular region; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; endopeptidase activity; caspase activator activity

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; membrane hyperpolarization; interleukin-1 beta production; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; mitochondrial depolarization; proteolysis; signal transduction; response to ATP; positive regulation of interleukin-1 alpha secretion; regulation of inflammatory response; response to hypoxia; innate immune response; regulation of autophagy