Rabbit anti-Human CASP6 Polyclonal Antibody | anti-CASP6 antibody

CASP6, NT (CASP6, MCH2, Caspase-6, Apoptotic protease Mch-2, Caspase-6 subunit p18, Caspase-6 subunit p11) (APC)

Cat. Num. AAA6280937

• Gene Names: CASP6; MCH2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CASP6; Polyclonal Antibody; NT (CASP6; MCH2; Caspase-6; Apoptotic protease Mch-2; Caspase-6 subunit p18; Caspase-6 subunit p11) (APC); Caspase-6 subunit p11; anti-CASP6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CASP6 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CASP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human CASP6.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CASP6 (arrow) using rabbit polyclonal CASP6 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP6 gene.)

Immunohistochemistry (IHC)

(CASP6 Antibody (N-term) (AP13835a)immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CASP6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CASP6 antibody

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in two transcript variants that encode different isoforms. [provided by RefSeq].

Product Categories/Family for anti-CASP6 antibody

Antibodies; Apoptosis; Caspase

References

Wurstle, M.L., et al. J. Biol. Chem. 285(43):33209-33218(2010). Lee, S.Y., et al. J Thorac Oncol 5(8):1152-1158(2010). Halawani, D., et al. J. Neurosci. 30(17):6132-6142(2010). Kim, M.S., et al. APMIS 118(4):308-312(2010). Yoo, N.J., et al. Tumori 96(1):138-142(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 14916485
• NCBI GeneID: 839
• NCBI Accession #: NP_116787.1; NP_001217.2
• NCBI GenBank Nucleotide #: NM_032992.2
• UniProt Accession #: P55212; Q9BQE7
• Molecular Weight: 22,574 Da
• NCBI Official Full Name: caspase-6 isoform beta
• NCBI Official Synonym Full Names: caspase 6
• NCBI Official Symbol: CASP6
• NCBI Official Synonym Symbols: MCH2
• NCBI Protein Information: caspase-6
• UniProt Protein Name: Caspase-6
• Protein Family: Caspase
• UniProt Gene Name: CASP6
• UniProt Synonym Gene Names: MCH2; CASP-6
• UniProt Entry Name: CASP6_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Oct 2015]

Uniprot Description

CASP6: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Activation is suppressed by phosphorylation at Ser-257. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.59; Protease; Apoptosis

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: epithelial cell differentiation; apoptosis; proteolysis; cell structure disassembly during apoptosis