Goat anti-Human Cartilage Intermediate-Layer Protein 1 Polyclonal Antibody | anti-CILP antibody

Cartilage Intermediate-Layer Protein 1 (NT) (CILP-1, CILP, Nucleotide pyrophosphohydrolase, HGNC:1980, HsT18872) (Biotin)

Cat. Num. AAA622948

• Gene Names: CILP; CILP-1; HsT18872
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: Cartilage Intermediate-Layer Protein 1; Polyclonal Antibody; Cartilage Intermediate-Layer Protein 1 (NT) (CILP-1; CILP; Nucleotide pyrophosphohydrolase; HGNC:1980; HsT18872) (Biotin); Anti -Cartilage Intermediate-Layer Protein 1 (NT) (CILP-1; anti-CILP antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CILP-1.
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from 0.2um filtered solution in PBS, BSA. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA. Labeled with Biotin.

• Applicable Applications for anti-CILP antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.1-0.2ug/ml
• Immunogen: NS0-derived recombinant human CILP-1 N-terminal peptide aa22-720.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CILP antibody

The CILP-1 (cartilage intermediate-layer protein 1) gene product is a 138kD monomeric glycoprotein that is found in both hyaline and fibrocartilage. It is a precursor for two secreted, proteolytically generated products, a 90kD N-terminal CILP-1, and a 62kD C-terminal NTPPHase-homolog. The N-terminus is suggested to serve as both a matrix structural protein, and an IGF-I/TGF-beta1 suppressor sequestration molecule. Human CILP-1 spans aa22-720 of the CILP-1 precursor. It contains one TSP-1 domain (aa149-201) and a C2-type Ig-like region (aa309-395). Over aa1-720 of the CILP-1 precursor, human CILP-1 shares 89% aa identity with mouse CILP-1, and 42% aa identity with human CILP-2.

Product Categories/Family for anti-CILP antibody

Antibodies; Abs to Extracellular Matrix Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 192449445
• NCBI GeneID: 8483
• NCBI Accession #: NP_003604.3
• NCBI GenBank Nucleotide #: NM_003613.3
• UniProt Accession #: O75339; Q6UW99; Q8IYI5; B2R8F7
• Molecular Weight: 132,565 Da
• NCBI Official Full Name: cartilage intermediate layer protein 1 preproprotein
• NCBI Official Synonym Full Names: cartilage intermediate layer protein, nucleotide pyrophosphohydrolase
• NCBI Official Symbol: CILP
• NCBI Official Synonym Symbols: CILP-1; HsT18872
• NCBI Protein Information: cartilage intermediate layer protein 1; OTTHUMP00000164180; cartilage intermediate-layer protein; cartilage intermediate layer protein 1 C1; cartilage intermediate layer protein 1 C2
• UniProt Protein Name: Cartilage intermediate layer protein 1
• Protein Family: Cartilage intermediate layer protein
• UniProt Gene Name: CILP
• UniProt Entry Name: CILP1_HUMAN

NCBI Description

Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq]

Uniprot Description

CILP: Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. Defects in CILP are a cause of susceptibility to intervertebral disk disease (IDD). A common musculo- skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Susceptibility to intervertebral disk disease, is conferred by variant p.Ile395Thr (PubMed:15864306).

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix

Molecular Function: nucleotide diphosphatase activity; alkaline phosphatase activity

Biological Process: negative regulation of insulin-like growth factor receptor signaling pathway

Disease: Intervertebral Disc Disease