Rabbit CARKL Polyclonal Antibody | anti-CARKL antibody

CARKL (A53) Polyclonal Antibody

Cat. Num. AAA3002516

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CARKL; Polyclonal Antibody; CARKL (A53) Polyclonal Antibody; A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95; AKAP8; AKAP95; anti-CARKL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CARKL pAb detects endogenous levels of CARKL protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 478

• Applicable Applications for anti-CARKL antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 333-393 of Human AKAP 95.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CARKL (A53) pAb at 1:500 dilutionLane1:The Kidney tissue lysate of Mouse (10ug) Lane2:The Kidney tissue lysate of Rat (10ug) Lane3:Panc1 whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug))

Related Product Information for anti-CARKL antibody

CARKL, also known as SHPK (sedoheptulokinase), is a 478 amino acid protein that localizes to the cytoplasm and belongs to the FGGY family of protein kinases. Expressed at high levels in kidney, pancreas and liver and at lower levels in heart, placenta, brain and lung, CARKL functions at an optimal pH of 8.5 and catalyzes the ATP-dependent phosphorylation of sedoheptulose to yield sedoheptulose 7-phosphate, an intermediate in the pentose phosphate pathway. Once phosphorylated, sedoheptulose is unable to exit the cell via the cell membrane, resulting in the containment of sedoheptulose 7-phosphate within the cell. Defects in the gene encoding CARKL are associated with cystinosis, an autosomal recessive genetic disorder of the renal tubules that is characterized by excessive urination and low blood levels of phosphates and potassium.

NCBI and Uniprot Product Information

• NCBI GI #: 74315356
• NCBI Accession #: NP_037408.2
• NCBI GenBank Nucleotide #: NP_037408.2
• UniProt Accession #: Q9UHJ6
• Molecular Weight: ~ 51kDa
• NCBI Official Full Name: sedoheptulokinase
• UniProt Protein Name: Sedoheptulokinase
• UniProt Gene Name: SHPK
• UniProt Synonym Gene Names: CARKL; SHK
• UniProt Entry Name: SHPK_HUMAN

Uniprot Description

SHPK: has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences, frequently involved in chromosomal rearrangements. [provided by RefSeq, Jul 2008]

Protein type: Kinase, other; EC 2.7.1.14

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: cytoplasm

Molecular Function: sedoheptulokinase activity; ATP binding

Biological Process: regulation of macrophage activation; carbohydrate metabolic process; regulation of inflammatory response; pentose-phosphate shunt, non-oxidative branch; phosphorylation