Western Blot (WB)
(Western blot analysis in CEM cell line lysates (35ug/lane) using MBS648011. This demonstrates the OPTN antibody detected the OPTN protein (arrow).)
Rabbit anti-Human OPTN Polyclonal Antibody | anti-OPTN antibody
OPTN, ID (OPTN, FIP2, GLC1E, HIP7, HYPL, NRP, Optineurin, E3-14.7K-interacting protein, FIP-2, Huntingtin yeast partner L, Huntingtin-interacting protein 7, Huntingtin-interacting protein L, NEMO-related protein, Optic neuropathy-inducing protein, Transcr
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(Western blot analysis in CEM cell line lysates (35ug/lane) using MBS648011. This demonstrates the OPTN antibody detected the OPTN protein (arrow).)
Western Blot (WB)
(Western blot analysis in CEM cell line lysates (35ug/lane) using MBS648011. This demonstrates the OPTN antibody detected the OPTN protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Ref.2 Ref.14 Ref.17
Subunit structure: Interacts with E3 14.7 kDa protein of group C human adenovirus. Interacts with HD. Interacts with Rab8 (RAB8A and/or RAB8B). Interacts with transcription factor IIIA (GTF3A). Interacts with TRAF3, TBK1 and MYO6. Binds to ubiquitin. Ref.1 Ref.8 Ref.9 Ref.11 Ref.14 Ref.17
Subcellular location: Cytoplasm › perinuclear region. Golgi apparatus. Golgi apparatus › trans-Golgi network. Note: Found in the perinuclear region and associates with the Golgi apparatus. Colocalizes with MYO6 and RAB8 at the Golgi complex and in vesicular structures close to the plasma membrane. Ref.1 Ref.2 Ref.10 Ref.14 Ref.17 Ref.28
Tissue specificity: Present in aqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta. Ref.1 Ref.2
Induction: Upon TNF and interferon treatments. Up-regulated in direct response to viral infection. Ref.1 Ref.10 Ref.12 Ref.13 Ref.17
Domain: Ubiquitin-binding motif (UBAN) is essential for its inhibitory function, subcellular localization and interaction with TBK1.
Post-translational modification: Phosphorylated by TBK1, leading to restrict bacterial proliferation in case of infection. Phosphorylation is induced by phorbol esters and decreases its half-time. Ref.10 Ref.29
Involvement in disease: Glaucoma 1, open angle, E (GLC1E) [MIM:137760]: A form of primary open angle glaucoma (POAG). POAG is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. However, glaucoma can occur at any intraocular pressure. The disease is generally asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2 Ref.21 Ref.22 Ref.24 Ref.25 Ref.26Glaucoma, normal pressure (NPG) [MIM:606657]: A primary glaucoma characterized by intraocular pression consistently within the statistically normal population range.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.27Amyotrophic lateral sclerosis 12 (ALS12) [MIM:613435]: A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.28
Caution: According to some authors (Ref.12) its expression is regulated by intraocular pressure, suggesting a protective role in case of high pressure, while according to other authors (Ref.13), it is not up-regulated in response to pressure elevation.
Sequence caution: The sequence CAI16552.1 differs from that shown. Reason: Erroneous gene model prediction.
Research Articles on OPTN
Similar Products
Product Notes
The OPTN optn (Catalog #AAA648011) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OPTN, ID (OPTN, FIP2, GLC1E, HIP7, HYPL, NRP, Optineurin, E3-14.7K-interacting protein, FIP-2, Huntingtin yeast partner L, Huntingtin-interacting protein 7, Huntingtin-interacting protein L, NEMO-related protein, Optic neuropathy-inducing protein, Transcr reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OPTN can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the OPTN optn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OPTN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
