Human Cardiolipin Polyclonal Antibody
Cardiolipin
Applications
ELISA
Purity
Purified by delipidation, defibrination and ammonium sulfate precipitation.
Synonyms
Cardiolipin; Polyclonal Antibody; Anti -Cardiolipin; anti-Cardiolipin antibody
Host
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Cardiolipin
Purity/Purification
Purified by delipidation, defibrination and ammonium sulfate precipitation.
Form/Format
Supplied as a lyophilized powder from PBS, 0.02% sodium azide.
Reconstitute with 2ml sterile ddH2O.
Reconstitute with 2ml sterile ddH2O.
Applicable Applications for anti-Cardiolipin antibody
Suitable for use in ELISA. Other applications have not been tested.
Application Notes
Recommended Dilutions:
ELISA: 1:100. Optimal dilutions to be determined by the researcher.
Autoantibody Activity:
Anti-Cardiolipin Activity by ELISA: As reported (lot-specific).
Current lot value: 13GPL/ml
Interpretation of Results: >12GPL/ml: Positive
Other Autoantibody Activity in the Serum (by ELISA):
SSA: 33U/ml
SSB: 9EU/ml
SCL: 18EU/ml
SM: 7EU/ml
Sm/RNP: 11U/ml
Jo-1: 31EU/ml
Interpretation of ELISA Results:
<16 EU/ml: Negative; 16-20 EU/ml: Equivocal; Positive; >20 EU/ml
ELISA: 1:100. Optimal dilutions to be determined by the researcher.
Autoantibody Activity:
Anti-Cardiolipin Activity by ELISA: As reported (lot-specific).
Current lot value: 13GPL/ml
Interpretation of Results: >12GPL/ml: Positive
Other Autoantibody Activity in the Serum (by ELISA):
SSA: 33U/ml
SSB: 9EU/ml
SCL: 18EU/ml
SM: 7EU/ml
Sm/RNP: 11U/ml
Jo-1: 31EU/ml
Interpretation of ELISA Results:
<16 EU/ml: Negative; 16-20 EU/ml: Equivocal; Positive; >20 EU/ml
Immunogen
None. This product is Cardiolipin auto-antibody derived from single donor human serum.
Grade
Purified
Preparation and Storage
Lyophilized and reconstituted products are stable after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Cardiolipin antibody
Cardiolipin is an important component of the inner mitochondrial membrane, where it constitutes about 20% of the total lipid composition. The name 'cardiolipin' is derived from the fact that it was first found in beef heart in the early 1940s. In mammalian cells, cardiolipin (CL) is found almost exclusively in the inner mitochondrial membrane where it is essential for the optimal function of numerous enzymes that are involved in mitochondrial energy metabolism.
Product Categories/Family for anti-Cardiolipin antibody
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Product Notes
The Cardiolipin (Catalog #AAA613746) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cardiolipin can be used in a range of immunoassay formats including, but not limited to, Suitable for use in ELISA. Other applications have not been tested. Recommended Dilutions: ELISA: 1:100. Optimal dilutions to be determined by the researcher. Autoantibody Activity: Anti-Cardiolipin Activity by ELISA: As reported (lot-specific). Current lot value: 13GPL/ml Interpretation of Results: >12GPL/ml: Positive Other Autoantibody Activity in the Serum (by ELISA): SSA: 33U/ml SSB: 9EU/ml SCL: 18EU/ml SM: 7EU/ml Sm/RNP: 11U/ml Jo-1: 31EU/ml Interpretation of ELISA Results: <16 EU/ml: Negative; 16-20 EU/ml: Equivocal; Positive; >20 EU/ml. Researchers should empirically determine the suitability of the Cardiolipin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cardiolipin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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