Rabbit anti-Human CARD9 Polyclonal Antibody | anti-CARD9 antibody

CARD9 Antibody

Cat. Num. AAA150414

• Gene Names: CARD9; CANDF2; hCARD9
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: CARD9 Antibody is affinity chromatography purified via peptide column.
• Synonyms: CARD9; Polyclonal Antibody; CARD9 Antibody; CANDF2; hCARD9; Caspase recruitment domain-containing protein 9; caspase recruitment domain family; member 9; anti-CARD9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: CARD9 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 2132

• Applicable Applications for anti-CARD9 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: CARD9 antibody can be used for detection of CARD9 by Western blot at 2.5 mug/mL. A band at approximately 59 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CARD9 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CARD9.
• Buffer: CARD9 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CARD9 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 antibody at 2.5 μg /ml. )

Immunocytochemistry (IHC)

(Immunocytochemistry of CARD9 in K562 cells with CARD9 antibody at 10 μg/mL.)

Related Product Information for anti-CARD9 antibody

CARD9 Antibody: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10. CARD9 associates with BCL-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-κB. CARD9 is an upstream activator of BCL-10 and NF-κB signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 11066983
• NCBI GeneID: 64170
• UniProt Accession #: Q9H257; Q5SXM5; Q5SXM6; Q9H854
• Molecular Weight: 59 kDa
• NCBI Official Full Name: Homo sapiens caspase recruitment domain protein 9 mRNA, complete cds
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 9
• NCBI Official Symbol: CARD9
• NCBI Official Synonym Symbols: CANDF2; hCARD9
• NCBI Protein Information: caspase recruitment domain-containing protein 9
• UniProt Protein Name: Caspase recruitment domain-containing protein 9
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD9
• UniProt Synonym Gene Names: hCARD9
• UniProt Entry Name: CARD9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the CARD protein family, which is defined by the presence of a characteristic caspase-associated recruitment domain (CARD). CARD is a protein interaction domain known to participate in activation or suppression of CARD containing members of the caspase family, and thus plays an important regulatory role in cell apoptosis. This protein was identified by its selective association with the CARD domain of BCL10, a postive regulator of apoptosis and NF-kappaB activation, and is thought to function as a molecular scaffold for the assembly of a BCL10 signaling complex that activates NF-kappaB. Several alternatively spliced transcript variants have been observed, but their full-length nature is not clearly defined. [provided by RefSeq, Jul 2008]

Uniprot Description

CARD9: Activates NF-kappa-B via BCL10. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2). Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytoplasm

Molecular Function: protein homodimerization activity; CARD domain binding

Biological Process: response to drug; response to fungus; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of interleukin-2 biosynthetic process; regulation of interleukin-6 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of tumor necrosis factor production; positive regulation of stress-activated MAPK cascade; regulation of apoptosis; defense response to Gram-positive bacterium; response to exogenous dsRNA; response to muramyl dipeptide; response to peptidoglycan; innate immune response; defense response to virus; regulation of tumor necrosis factor biosynthetic process

Disease: Candidiasis, Familial, 2