Rabbit anti-Human CARD8 / Cardinal / TUCAN Polyclonal Antibody | anti-CARD8 antibody

Rabbit Polyclonal to Human CARD8 / Cardinal / TUCAN

Cat. Num. AAA240458

• Gene Names: CARD8; NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: CARD8 / Cardinal / TUCAN; Polyclonal Antibody; Rabbit Polyclonal to Human CARD8 / Cardinal / TUCAN; Anti-CARD8 / Cardinal / TUCAN Antibody (C-Terminus) IHC-plus; CARD8; Apoptotic protein NDPP1; DAKAR; KIAA0955; NDPP; TUCAN; CARDINAL; DACAR; NDPP1; Human CARD8; Cardinal; anti-CARD8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: synthetic peptide corresponding to amino acids at the C-terminus of human CARD8
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 431

• Applicable Applications for anti-CARD8 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: ICC, IHC-P (5 ug/ml), WB (2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human CARD8.
• Immunogen Type: Synthetic peptide
• Immunogen: CARD8 / Cardinal / TUCAN antibody was raised against synthetic peptide from human CARD8.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-CARD8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 ug/ml.)

Western Blot (WB)

(Western blot of CARD8 expression in K562 cell lysate with CARD8 antibody at (A) 2 and (B) 4 ug /ml.)

Related Product Information for anti-CARD8 antibody

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8.

Product Categories/Family for anti-CARD8 antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 7662404
• NCBI GeneID: 22900
• NCBI Accession #: NP_055774.1
• NCBI GenBank Nucleotide #: NM_014959.2
• UniProt Accession #: Q9Y2G2; Q6PGP8; Q96P82; B5KVR6; B7Z496; B7Z4A2; E9PEM7; G3XAM9
• Molecular Weight: 60,652 Da
• NCBI Official Full Name: caspase recruitment domain-containing protein 8
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 8
• NCBI Official Symbol: CARD8
• NCBI Official Synonym Symbols: NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL
• NCBI Protein Information: caspase recruitment domain-containing protein 8; apoptotic protein NDPP1; CARD inhibitor of NF-kappaB-activating ligands; tumor up-regulated CARD-containing antagonist of CASP9; tumor up-regulated CARD-containing antagonist of caspase nine
• UniProt Protein Name: Caspase recruitment domain-containing protein 8
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD8
• UniProt Synonym Gene Names: KIAA0955; NDPP1; CARDINAL; TUCAN
• UniProt Entry Name: CARD8_HUMAN

NCBI Description

The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

CARD8: Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Apoptosis

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; NACHT domain binding; protein homodimerization activity; caspase activator activity

Biological Process: caspase activation; positive regulation of caspase activity; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 beta secretion