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Western Blot (WB) (Fig : 1 specifically recognizes NLRCR4, but not closely related NLR family members NOD1 and NOD2. HEK293T cells transiently transfected with with human NOD1, NOD or NLRC4 c-myc plasmids were IP'd with a c-myc antibody followed by Fig: 1 Western blot with the NLRC4 antibody. Data courtesy of Dr Roland Wagner, Sanford-Burnham Medical Institute.)

Rabbit CARD12 Polyclonal Antibody | anti-CARD12 antibody

Polyclonal Antibody to CARD12 (IPAF/ClAN)

Gene Names
NLRC4; CLAN; IPAF; AIFEC; CLAN1; CLANA; CLANB; CLANC; CLAND; FCAS4; CARD12; CLR2.1
Reactivity
Human
Predicted: Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Serum
Synonyms
CARD12; Polyclonal Antibody; Polyclonal Antibody to CARD12 (IPAF/ClAN); NLRC4; CLAN; CLAN1; IPAF; UNQ6189/PRO20215; CARD; LRR; and NACHT-containing protein; Caspase recruitment domain-containing protein 12; Ipaf; Ice protease-activating factor; Antibody to CARD12 (IPAF/ClAN); Anti-CARD12 (IPAF/ClAN) Antibody; CARD12 (IPAF/ClAN) Antibody; anti-CARD12 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Predicted: Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Serum
Form/Format
50 ul sera
Sequence Length
1024
Applicable Applications for anti-CARD12 antibody
Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP)
Application Notes
WB: 1: 1000-1: 2000
IHC (paraffin): 1: 1000-1: 5000
IP: 1: 50-1: 200
Immunogen Information
A synthetic peptide of human IPAF/ClAN/CARD12 (amino acids 971-989 DFSTKEFLPDPALVRKLSQ) was used as immunogen for this antibody
Antigen
CARD12 (IPAF/ClAN)
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.

Western Blot (WB)

(Fig : 1 specifically recognizes NLRCR4, but not closely related NLR family members NOD1 and NOD2. HEK293T cells transiently transfected with with human NOD1, NOD or NLRC4 c-myc plasmids were IP'd with a c-myc antibody followed by Fig: 1 Western blot with the NLRC4 antibody. Data courtesy of Dr Roland Wagner, Sanford-Burnham Medical Institute.)

Western Blot (WB) (Fig : 1 specifically recognizes NLRCR4, but not closely related NLR family members NOD1 and NOD2. HEK293T cells transiently transfected with with human NOD1, NOD or NLRC4 c-myc plasmids were IP'd with a c-myc antibody followed by Fig: 1 Western blot with the NLRC4 antibody. Data courtesy of Dr Roland Wagner, Sanford-Burnham Medical Institute.)
Related Product Information for anti-CARD12 antibody
Ipaf (also known as Clan/CARD12/NLRC4) is a CARD domain containing protein belongs to the NLR family. In general CARD proteins are implicated in host defense against infection, environmental stress or cellular damage. CARD domains are homotypic protein interaction motifs that enable networks of proteins to communicate via CARD-CARD interactions. There are at least three major signaling pathways in which CARD proteins act: (1) Regulation of caspase activation in the context of apoptosis (2) Regulation of caspase activation in the context of inflammation (3) Regaultion of NF-kB activation in the context of innate or adaptive immune responses. Ipaf plays a role in regulating caspase-1 activity, which in turn mediates the maturation of inflammatory cytokines IL-1b and IL-18. In transfected cells, Ipaf has been shown to directly interact with procaspase-1 and induce proteolytic activation of procaspase-1 in transfected cells. On the flip side, macrophages from IPAF deficient mice failed to activate caspase-1 in response to Salmonella typhimurium infection underscoring the importance of IPAF in vivo. IPAF also interact with the pro-apoptotic adaptor protein ASC and co-expression of IPAF with ASC has been shown to induce NF-kB activation and apoptosis.
Product Categories/Family for anti-CARD12 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
10,646 Da
NCBI Official Full Name
NLR family CARD domain-containing protein 4
NCBI Official Synonym Full Names
NLR family CARD domain containing 4
NCBI Official Symbol
NLRC4
NCBI Official Synonym Symbols
CLAN; IPAF; AIFEC; CLAN1; CLANA; CLANB; CLANC; CLAND; FCAS4; CARD12; CLR2.1
NCBI Protein Information
NLR family CARD domain-containing protein 4
UniProt Protein Name
NLR family CARD domain-containing protein 4
UniProt Gene Name
NLRC4
UniProt Synonym Gene Names
CARD12; CLAN; CLAN1; IPAF; Clan protein; Ipaf

NCBI Description

This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

NLRC4: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 2p22.3

Cellular Component: cytosol; nucleus; spindle microtubule

Molecular Function: ATP binding; cysteine protease inhibitor activity; identical protein binding; magnesium ion binding; protein binding; protein homodimerization activity; ubiquitin-protein ligase activity

Biological Process: activation of innate immune response; activation of NF-kappaB transcription factor; caspase activation; defense response to bacterium; detection of bacterium; inflammatory response; innate immune response; interleukin-1 beta secretion; positive regulation of apoptosis; protein homooligomerization; regulation of apoptosis; regulation of signal transduction

Disease: Autoinflammation With Infantile Enterocolitis; Familial Cold Autoinflammatory Syndrome 4

Research Articles on CARD12

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Product Notes

The CARD12 nlrc4 (Catalog #AAA668562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to CARD12 (IPAF/ClAN) reacts with Human Predicted: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CARD12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP). WB: 1: 1000-1: 2000 IHC (paraffin): 1: 1000-1: 5000 IP: 1: 50-1: 200. Researchers should empirically determine the suitability of the CARD12 nlrc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARD12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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