Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Mouse anti-Human, Rat SH3MD2 Monoclonal Antibody | anti-sh3rf1 antibody
SH3MD2 (SH3RF1, Putative E3 Ubiquitin-protein Ligase SH3RF1, SH3 Domain-containing RING Finger Protein 1, Plenty of SH3s, Protein POSH, SH3 Multiple Domains Protein 2, RING Finger Protein 142, KIAA1494, POSH, RNF142)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Western Blot (WB)
(SH3MD2 monoclonal antibody, Western Blot analysis of SH3MD2 expression in HeLa.)
Western Blot (WB)
(SH3MD2 monoclonal antibody, Western Blot analysis of SH3MD2 expression in HeLa.)
Western Blot (WB)
(SH3MD2 monoclonal antibody. Western Blot analysis of SH3MD2 expression in PC-12.)
Western Blot (WB)
(SH3MD2 monoclonal antibody. Western Blot analysis of SH3MD2 expression in PC-12.)
Testing Data
(Detection limit for recombinant GST tagged SH3MD2 is ~0.03ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged SH3MD2 is ~0.03ng/ml as a capture antibody.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (mapk8 and mapk9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as map3k10 or map3k11 which are in turn activated leading to the sequential activation of map2k4, map2k7 and JNKs (mapk8 and mapk9)
By similarity. Plays an essential role in the anterior neural development.Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes such as ube2d1 or ube2n and then transfers it to substrates. In the absence of an external substrate, it can catalyze self-ubiquitination
By similarity.
Pathway: Protein modification; protein ubiquitination.
Subcellular location: Cytoplasm › perinuclear region
By similarity. Cell projection › lamellipodium
By similarity. Golgi apparatus › trans-Golgi network
By similarity.
Domain: The RING finger domain is responsible of ubiquitination and proteasomal degradation.
Post-translational modification: Subject to ubiquitination and proteasomal degradation
By similarity.
Sequence similarities: Belongs to the SH3RF family.Contains 1 RING-type zinc finger.Contains 4 SH3 domains.
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Product Notes
The sh3rf1 sh3rf1 (Catalog #AAA6004503) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SH3MD2 (SH3RF1, Putative E3 Ubiquitin-protein Ligase SH3RF1, SH3 Domain-containing RING Finger Protein 1, Plenty of SH3s, Protein POSH, SH3 Multiple Domains Protein 2, RING Finger Protein 142, KIAA1494, POSH, RNF142) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SH3MD2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the sh3rf1 sh3rf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LAQDAFHRKA SSLDSAVPIA PPPRQACSSL GPVLNESRPV VCERHRVVVS YPPQSEAELE LKEGDIVFVH KKREDGWFKG TLQRNGKTGL FPGSFVENI. It is sometimes possible for the material contained within the vial of "SH3MD2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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