CAR/CXADR Recombinant Protein | CXADR recombinant protein

CAR/CXADR Protein, Mouse, Recombinant (ECD, His & hFc Tag)

Cat. Num. AAA8122468

• Gene Names: Cxadr; CAR; MCAR; MCVADR; AU016810; AW553441; 2610206D03Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CAR/CXADR; CAR/CXADR Protein; Mouse; Recombinant (ECD; His & hFc Tag); Mouse CXADR/CAR Protein (ECD; Fc & His Tag); 2610206D03Rik Protein; AU016810 Protein; AW553441 Protein; CAR Protein; MCAR Protein; MCVADR Protein; coxsackie virus and adenovirus receptor; CXADR recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly237

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Leu 20
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the mouse Cxadr (NP_001020363.1)(Met1-Gly237) was expressed with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus (Fc-his).
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CXADR recombinant protein

Background: CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 17 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 9% sequence identity with the corresponding regions of rat, human CXADR.

References

Tomko, R.P. et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94 (7): 3352-3356. van Raaij, M.J. et al., 2001, Structure. 8 (11): 1147-1155. Cohen, C.J. et al., 2001, J. Biol. Chem. 276 (27): 25392-25398. Carson, S.D. et al., 2002, Rev. Med. Virol. 11 (4): 219-226. Selinka, H.C. et al., 2004, Med. Microbiol. Immunol. 193 (2-3): 127-131. Philipson, L. et al., 2004, Curr. Top. Microbiol. Immunol. 273:87-111. Raschperger, E. et al., 2006, Exp. Cell Res. 312: 1566-1580.

NCBI and Uniprot Product Information

• NCBI GI #: 68510034
• NCBI GeneID: 13052
• NCBI Accession #: NP_001020363.1
• NCBI GenBank Nucleotide #: NM_001025192.3
• UniProt Accession #: P97792; O09052; Q3ULD0; Q91W66; Q99KG0; Q9DBJ8
• Molecular Weight: 17,781 Da
• NCBI Official Full Name: coxsackievirus and adenovirus receptor homolog isoform a
• NCBI Official Synonym Full Names: coxsackie virus and adenovirus receptor
• NCBI Official Symbol: Cxadr
• NCBI Official Synonym Symbols: CAR; MCAR; MCVADR; AU016810; AW553441; 2610206D03Rik
• NCBI Protein Information: coxsackievirus and adenovirus receptor homolog
• UniProt Protein Name: Coxsackievirus and adenovirus receptor homolog
• Protein Family: Coxsackievirus and adenovirus receptor
• UniProt Gene Name: Cxadr
• UniProt Synonym Gene Names: Car; CAR; mCAR

NCBI Description

This gene encodes a protein that is part of the Cortical Thymocyte marker in Xenopus (CTX) subfamily within the immunoglobulin superfamily. Members of this subfamily, predominantly expressed on the surface of endothelial and epithelial cells, help establish cell polarity and provide a barrier function, regulating migration of immune cells. This protein, first identified as the receptor for adenovirus subgroup C and coxsakieviruses group B, is developmentally regulated and plays an important role in cardiac development. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Uniprot Description

Component of the epithelial apical junction complex that may function as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.