Rabbit CaMKIalpha Polyclonal Antibody | anti-CaMKIalpha antibody

CaMKIalpha (S173) Polyclonal Antibody

Cat. Num. AAA3001581

• Gene Names: CAMK1; CAMKI
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CaMKIalpha; Polyclonal Antibody; CaMKIalpha (S173) Polyclonal Antibody; CaM K1; CaM KI; CaM kinase 1 alpha; CAM kinase 1; CaM kinase I alpha; CAM kinase I; CaMK 1; CAMK I; CaMK1 alpha; CaMK1; CAMK1 PEN; CaMKI alpha; MGC120317; MGC120318; anti-CaMKIalpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CaMKIalpha (S173) polyclonal antibody detects endogenous levels of CaMKIalpha protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 370

• Applicable Applications for anti-CaMKIalpha antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 140-192 of Human CaMKIalpha.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of CaMKIalpha (S173) pAb in paraffin-embedded human colorectal cancer carcinoma tissue at 1:100.)

Related Product Information for anti-CaMKIalpha antibody

The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated alpha, beta, gamma and delta, which may or may not be coexpressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal, leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory threonine residue at position 177.

NCBI and Uniprot Product Information

• NCBI GI #: 4502553
• NCBI GeneID: 8536
• NCBI Accession #: NP_003647.1
• NCBI GenBank Nucleotide #: NP_003647.1
• UniProt Accession #: Q14012
• Molecular Weight: ~ 41kDa
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type 1
• NCBI Official Synonym Full Names: calcium/calmodulin dependent protein kinase I
• NCBI Official Symbol: CAMK1
• NCBI Official Synonym Symbols: CAMKI
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type 1
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type 1
• UniProt Gene Name: CAMK1
• UniProt Synonym Gene Names: CaM-KI; CaMKI-alpha
• UniProt Entry Name: KCC1A_HUMAN

NCBI Description

Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMK1A: an ubiquitous protein kinase of the CAMK1 family. Activated by Ca(2+)/calmodulin. Must be phosphorylated to be maximally active. Substrates include synapsin I.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.17; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytoplasm; nucleus

Molecular Function: calmodulin binding; protein binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: positive regulation of syncytium formation by plasma membrane fusion; regulation of muscle cell differentiation; nucleocytoplasmic transport; signal transduction; cell cycle; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein export from nucleus; positive regulation of synapse structural plasticity; regulation of protein localization; regulation of protein binding; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein binding